Methylated DNA Immunoprecipitation (MeDIP) Kit - Tissue (ab117136)

製品の概要

  • 製品名Methylated DNA Immunoprecipitation (MeDIP) Kit - Tissue
    hydroxymethylated DNA キット 製品一覧
  • サンプルの種類
    Tissue
  • 全工程の試験時間
    3h 30m
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 製品の概要

    DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer.

     Methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117135) enables the user to enrich methylated DNA directly from various mammalian tissues by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.

  • アプリケーション適用あり: Functional Studiesmore details

製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab117136 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    Functional Studies Use at an assay dependent concentration.

    プロトコール

    Methylated DNA Immunoprecipitation (MeDIP) Kit - Tissue (ab117136) 使用論文

    ab117136 has not yet been referenced specifically in any publications.

    Product Wall

    I apologize again for the incorrect information given originally.

    For tissues we recommend, ab117136.
    In comparison, kit ab117135 would be used when starting with cells. When using a DNA sample, please use ab117133 instead.

    I ...

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    The ChiPed DNA amount with this kit is about 30-50 ng/well. Genome-wide amplification may be needed for some downstream applications such as microarray or sequencing. I hope this is helpful. Please let me know if you need anything further.

    I am sorry for the delay in our reply. Here are answers to your questions: 1. The antibodies included in ab117136, 117144, 117145, 117147, 117150 and117151 are not sold separately in our catalog. 2. The antibodies in kits ab117144, 117145 and117147 hav...

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    1. Yes, the ChIPed DNA is suitable for ChIP-Seq. Some customers report having improved ChIP-Seq results when the ChIPed DNA is further purified via phenol/chloroform extraction and ethanol precipitation. 2. The 'required volume' is unfortunately vague ...

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    This kit can be used for ChIP with adipose tissues. Here are the references for using our general tissue ChIP kit in adipose tissues: - Yue, L.et. al.(April 2009). Peroxisome proliferator-activated receptor {gamma} stimulation of adipocyte ApoE gene tr...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"