Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (ab117133)


  • 製品名
    Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA
    5-methylcytosine キット 製品一覧
  • 全工程の試験時間
    3h 00m
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, a wide range of other species
  • 製品の概要

    DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer.

    Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (ab117133) enables the user to enrich methylated DNA by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.

  • 特記事項

    NOTE: the size of this kit is based on number of TESTS not SAMPLES. A test simply refers to a single assay well. If you are not sure which size is most suitable for you, contact our Scientific Support Team.

  • アプリケーション
    適用あり: Functional Studiesmore details



Our Abpromise guarantee covers the use of ab117133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.


  • MeDIP was performed using 1μg of DNA with ab117133.  Real-time PCR was carried out with MeDIP DNAs and the frequency of DNA methylation of immunoprecipitated DNA vs input DNA was calculated.

  • Captured methylated DNA was used for analyzing methylation level of GAPDH and MLH1 promoter with the use of primers and probes specific to GAPDH and MLH1 promoters, respectively.
  • For enrichment of methylated DNA using ab117133, DNA (0.5 ug) isolated from MCF-7 cells was added into the microwell. Methylated DNA was captured by 5-mC antibody prebound to the microwells.



This product has been referenced in:
  • Han B  et al. Variation of DNA Methylome of Zebrafish Cells under Cold Pressure. PLoS One 11:e0160358 (2016). MeDIP . Read more (PubMed: 27494266) »
  • Schultz BM  et al. Enhancers compete with a long non-coding RNA for regulation of the Kcnq1 domain. Nucleic Acids Res 43:745-59 (2015). MeDIP ; Mouse . Read more (PubMed: 25539921) »

See all 2 Publications for this product

レビューと Q&A

Both of these kits (ab117133 and ab117135) will immunoprecipitate methylated DNA. The ab117133 Kit uses purified DNA as starting materials while the ab117135 Kit uses cells as starting materials. I can confirm that the immunoprecipitated products are s...

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Here are the sequences for human H19ICR and GAPDH:

H19ICR(234 bp length of amplicons):


GAPDH (110 bp length of amplicons)


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Thank you for your inquiry.

With use of this kit, about 40 ng/well of meDNA can be generated from 500 ng of inputgenomic DNA. Thus, at least 20 ug of genomic DNA (in 40 wells) are needed for generating 1.5 ug of methylated DNA.

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Thank you for your inquiry.

You will need 1 well per plate dedicated to the negative control if you're running all at the same time. So for 60 samples you'd probably need to orderone 48 sample kit and one 24 sample kit. The kits are in strip ...

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Thank you for contacting us.

The pre-sonicated DNA sample can be used if the DNA is in the buffer(such as TE containing low concentration of salts) that would not interfereantibody binding to the meDNA, or use MC2 to ...

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Thank you for contacting us.
The elution buffer EMD6 used is RNAse/DNAse free water.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an ...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one EpiSeeker methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (ab117133). Read More

I apologize again for the incorrect information given originally.

For tissues we recommend, ab117136.
In comparison, kit ab117135 would be used when starting with cells. When using a DNA sample, please use ab117133 instead.

I ...

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