Anti-Methotrexate 抗体 (ab2328)

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Customer reviews and FAQs

製品の概要

  • 製品名Anti-Methotrexate antibody
  • 製品の詳細
    Goat polyclonal to Methotrexate
  • 特異性The antibody is highly specific for Methotrexate, but cross-reactivities have not been tested.
  • アプリケーション適用あり: ELISA, RIAmore details
  • 免疫原

    Pure Methotrexate conjugated to hemocyanin

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
RIA
  • 追加情報Working dilutions are best determined by the end user.

    • ターゲット情報

    • 関連性Methotrexate (rINN) abbreviated MTX and formerly known as amethopterin, is an antimetabolite and antifolate drug used in treatment of cancer, autoimmune diseases and as an abortifacient in the induction of medical abortions. It acts by inhibiting the metabolism of folic acid. Methotrexate competitively inhibits dihydrofolate reductase (DHFR), an enzyme that participates in the tetrahydrofolate synthesis. The affinity of methotrexate for DHFR is about one thousand-fold that of folate for DHFR. Methotrexate acts specifically during DNA and RNA synthesis, and thus it is cytotoxic during the S-phase of the cell cycle. Logically, it therefore has a greater toxic effect on rapidly dividing cells which replicate their DNA more frequently, and thus inhibits the growth and proliferation of these non-cancerous cells. Lower doses of methotrexate have been shown to be very effective for the management of rheumatoid arthritis, Crohn's disease, and psoriasis.
    • 別名
      • Amethopterin antibody
      • MTX antibody

    Anti-Methotrexate antibody (ab2328) 使用論文

    ab2328 has not yet been referenced specifically in any publications.

    ab2328 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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    ab2328 の abreview を投稿する ab2328 に関する問い合わせをする

    3 Abreviews or Q&A

    10
    Votes: Highest First

    1- you mentioned an estimate of the antibody concentration in mouse, however, the one that we are using is a goat methotrexate antibody ab2328 , so any estimate for this one would be helpful. 2- in the serum, only the specific antibody would bind to...

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    Thank you for your email and kind answer. Indeed, I have found the below publication where the IgG content of normal adult goat serum was about 20mg/ml. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1445503/ Also, yes, only the specific antibodie...

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    I have another question about anti-Methotrexate antibodies (ab2328). The product of ab2328 is the antiserum, so if I want to purify Methotrexate antibodies, do I need to use Pure Methotrexate conjugated to hemocyanin, the immunogen of the antibodies? C...

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    Thank you for your enquiry. I apologise for the delay in responding to your message. Since it is the antibody directed against methotrexate that you need to purify, you will not need to include the hemocyanin. IgG from the sample can be purified no...

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    Is this antibody part of a kit for doing ELISA?

    Thank you for your enquiry. This antibody is not part of a kit, but we do have suggestions for compatible secondary antibodies and other products that may be helpful. These are available on the "Related Products" tab on the datasheet. I hope thi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"