Anti-Methionine Aminopeptidase 2 抗体 [EPR6886(B)] (ab124953)


  • 製品名Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)]
    Methionine Aminopeptidase 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6886(B)] to Methionine Aminopeptidase 2
  • アプリケーション適用あり: WB, Flow Cyt, IHC-Frmore details
    適用なし: ICC/IF,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to residues in Human Methionine Aminopeptidase 2.

  • ポジティブ・コントロール
    • HepG2, 293T, HeLa, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates; Permeabilized 293T cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.



Our Abpromise guarantee covers the use of ab124953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 52 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
  • 追加情報Is unsuitable for ICC/IF,IHC-P or IP.
  • ターゲット情報

    • 機能Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
      Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
    • 配列類似性Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.
    • 翻訳後修飾Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding.
    • 細胞内局在Cytoplasm. About 30% of expressed METAP2 associates with polysomes.
    • Information by UniProt
    • 参照データベース
    • 別名
      • A930035J23Rik antibody
      • AI047573 antibody
      • AL024412 antibody
      • Amp2 antibody
      • AU014659, antibody
      • EIF 2 associated p67 homolog antibody
      • EIF-2-associated p67 antibody
      • Initiation factor 2 associated 67 kDa glycoprotein antibody
      • Initiation factor 2-associated 67 kDa glycoprotein antibody
      • MAP 2 antibody
      • MAP2 antibody
      • MAP2_HUMAN antibody
      • MetAP 2 antibody
      • Metap2 antibody
      • Methionine aminopeptidase 2 antibody
      • Methionyl aminopeptidase 2 antibody
      • MGC102452 antibody
      • MGC127390 antibody
      • MGC53792 antibody
      • MNPEP antibody
      • p67 antibody
      • p67eIF2 antibody
      • Peptidase M antibody
      • Peptidase M2 antibody
      see all

    Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)] 画像

    • All lanes : Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)] (ab124953) at 1/1000 dilution

      Lane 1 : HepG2 cell lysates
      Lane 2 : 293T cell lysates
      Lane 3 : HeLa cell lysates
      Lane 4 : C6 cell lysates
      Lane 5 : RAW 264.7 cell lysates
      Lane 6 : PC12 cell lysates
      Lane 7 : NIH 3T3 cell lysates

      Lysates/proteins at 10 µg per lane.

      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 52 kDa
      Observed band size : 67 kDa (why is the actual band size different from the predicted?)
    • ab124953 at 1/10 dilution staining Methionine Aminopeptidase 2 in permeabilized 293T cells by Flow cytometry (red) or a Rabbit IgG (negative control) (green).
    • ab124953 staining Methionine Aminopeptidase 2 in Mouse spleen tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with Acetone and blocked with 5% Serum for 60 minutes at 25°C. Samples were incubated with primary antibody (1/25 in PBS + 5% goat serum) for 1 hours at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

    Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)] (ab124953) 使用論文

    This product has been referenced in:
    • Ng JY  et al. Tyrosine nitration moderates the peptidase activity of human methionyl aminopeptidase 2. Biochem Biophys Res Commun 440:37-42 (2013). Read more (PubMed: 24041691) »

    See 1 Publication for this product

    Product Wall

    The immunogen for Methionine Aminopeptidase 2 antibody [EPR6886(B)] (ab124953) was within the first 50 amino acids of the N terminus. So, the antibody will not recognize the truncated protein that is missing the 107 N-terminal amino acids.

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Spleen from immune mouse)
    Specification Spleen from immune mouse
    Fixative Acetone
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    投稿 Jan 08 2013