Anti-Methionine Aminopeptidase 2 抗体 (ab123523)


  • 製品名Anti-Methionine Aminopeptidase 2 antibody
    Methionine Aminopeptidase 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Methionine Aminopeptidase 2
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Horse, Cow, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 100 to 150 of Human Methionine Aminopeptidase 2 (NP_006829.1)

  • ポジティブ・コントロール
    • HeLa, 293T, Jurkat, NIH3T3 whole cell lysates



Our Abpromise guarantee covers the use of ab123523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 52 kDa.
IP Use at 2-10 µg/mg of lysate.


  • 機能Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
    Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis.
  • 配列類似性Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.
  • 翻訳後修飾Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding.
  • 細胞内局在Cytoplasm. About 30% of expressed METAP2 associates with polysomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A930035J23Rik antibody
    • AI047573 antibody
    • AL024412 antibody
    • Amp2 antibody
    • AU014659, antibody
    • EIF 2 associated p67 homolog antibody
    • EIF-2-associated p67 antibody
    • Initiation factor 2 associated 67 kDa glycoprotein antibody
    • Initiation factor 2-associated 67 kDa glycoprotein antibody
    • MAP 2 antibody
    • MAP2 antibody
    • MAP2_HUMAN antibody
    • MetAP 2 antibody
    • Metap2 antibody
    • Methionine aminopeptidase 2 antibody
    • Methionyl aminopeptidase 2 antibody
    • MGC102452 antibody
    • MGC127390 antibody
    • MGC53792 antibody
    • MNPEP antibody
    • p67 antibody
    • p67eIF2 antibody
    • Peptidase M antibody
    • Peptidase M2 antibody
    see all

Anti-Methionine Aminopeptidase 2 antibody 画像

  • Detection of Methionine Aminopeptidase 2 by Western Blot of Immunprecipitate.
    Lane 1: ab123523 at 1µg/ml staining Methionine Aminopeptidase 2 in HeLa whole cell lysate immunoprecipitated using ab123523 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 10 seconds.
  • All lanes : Anti-Methionine Aminopeptidase 2 antibody (ab123523) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    developed using the ECL technique

    Predicted band size : 52 kDa

    Exposure time : 30 seconds

Anti-Methionine Aminopeptidase 2 antibody (ab123523) 使用論文

ab123523 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab123523.
Please use the links above to contact us or submit feedback about this product.