製品の概要

  • 製品名
    Metformin hydrochloride
  • 製品の詳細
    AMPK activator
  • 生理活性の詳細
    AMPK activator. Antidiabetic and anticancer activity in vivo. Rapidly crosses the blood-brain barrier.

    Also available in simple stock solutions (ab146725) - add 1 ml of water to get an exact, ready-to-use concentration.
  • 精製度
    > 99%

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab120847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Functional Studies Use at an assay dependent concentration.

画像

  • ab1 staining HIF1α in MCF7 cells treated with metformin hydrochloride (ab120847), by ICC/IF. Decrease in HIF1α expression correlates with increased concentration of metformin hydrochloride, as described in literature.
    The cells were incubated at 37°C for 24h in media containing different concentrations of ab120847 (metformin hydrochloride) in water, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

参考文献

This product has been referenced in:
  • Gallagher EJ & LeRoith D Diabetes, cancer, and metformin: connections of metabolism and cell proliferation. Ann N Y Acad Sci 1243:54-68 (2011). Read more (PubMed: 22211893) »
  • Labuzek K  et al. Quantification of metformin by the HPLC method in brain regions, cerebrospinal fluid and plasma of rats treated with lipopolysaccharide. Pharmacol Rep 62:956-65 (2010). Read more (PubMed: 21098880) »

See 1 Publication for this product

レビューと Q&A

There are currently no Customer reviews or Questions for ab120847.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

登録