Anti-Metallothionein 抗体 [UC1MT] (ab12228)

製品の概要

  • 製品名Anti-Metallothionein antibody [UC1MT]
    Metallothionein 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [UC1MT] to Metallothionein
  • 特異性Cross reacts with MT1 and MT2.
  • アプリケーション適用あり: IHC-Fr, ICC/IF, WB, Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Horse, Dog, Human, Mummichug fish
  • 免疫原

    Full length protein (cross linked rabbit liver MT1 and MT2).

  • ポジティブ・コントロール
    • HeLa cell lysate treated with 100uM CdCl2 Rehydrated rabbit liver MTI/MTII

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12228 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/1000. Detects a band of approximately 6-20 kDa (predicted molecular weight: 6 kDa). Please note: often Western blots done on cell lysates with this antibody produce many bands; we suspect that metallothionein binds to many other proteins, thus producing these results. As the predicted MW is around 6 kDa, use 12.5-20% gel and be sure the protein is not run off the gel during electrophoresis.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-P Use at an assay dependent concentration.

ターゲット情報

Anti-Metallothionein antibody [UC1MT] 画像

  • Anti-Metallothionein antibody [UC1MT] (ab12228) + Hela cell lysate

    Secondary
    HRP-conjugated antibody.
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 6 kDa


    Exposure time : 2 minutes
  • ab12228 staining catfish kidney tissue sections by IHC-Fr.  Sections were acetone fixed and blocked with a commercial blocking agent prior to incubation with the primary antibody, diluted 1/50, for 16 hours at 4°C.  An Alexa Fluor® 488 conjugated goat anti-mouse antibody, diluted 1/1000, was used as the secondary.

    See Abreview

  • Anti-Metallothionein antibody [UC1MT] (ab12228) at 1/1000 dilution + Rabbit liver lysates

    Predicted band size : 6 kDa
  • ICC/IF image of ab12228 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12228, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ICC/IF image of ab12228 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12228, 10µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Overlay histogram showing HeLA cells stained with ab12228 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12228, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • ab12228 staining human uterus tissue at 10 ug/ml using IHC-P.
  • All lanes : Anti-Metallothionein antibody [UC1MT] (ab12228)

    Lane 1 : Marker
    Lane 2 : MTI
    Lane 3 : MTII
    Lane 4 : Mummichug CdCl2


    Predicted band size : 6 kDa

Anti-Metallothionein antibody [UC1MT] (ab12228) 使用論文

This product has been referenced in:
  • Zhang D  et al. Zinc supplementation protects against cadmium accumulation and cytotoxicity in Madin-Darby bovine kidney cells. PLoS One 9:e103427 (2014). WB, ICC/IF ; Dog . Read more (PubMed: 25105504) »
  • Baba H  et al. The liver in itai-itai disease (chronic cadmium poisoning): pathological features and metallothionein expression. Mod Pathol 26:1228-34 (2013). IHC-P ; Human . Read more (PubMed: 23558578) »

See all 17 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (15% acrylamide)
Sample Human Cell lysate - whole cell (osteosarcoma)
Specification osteosarcoma
Blocking step blocking buffer Sigma as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
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投稿 Jan 14 2015

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10 · Temperature: 23°C
Sample Rat Tissue sections (Brain)
Specification Brain
Permeabilization Yes - Methanol
Fixative Methanol
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投稿 Oct 27 2014

Thank you for contacting us.

The reason it comes as an error message is because there is no specific protocol for this antibody. The link to the general protocol is below:

http://www.abcam.com/index.html?pageconfig=resource&rid=11...

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We do not have any free samples or small samples of any of our antibodies but both ab12228 and ab3125 will be covered by our guarantee for one year if you plan on using them for flow cytometry with samples that are one of the species listed on the data...

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Thank you for contacting us. Because we carry over 90,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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Thank you for your new contact.

My colleague is out of office today. I have attached the Western blot Questionnaire to this e-mail and would be grateful if you could complete it and send it back to us.

Thank you for your understandi...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and will be very happy to investigate the antibody further.

- Please fill the ...

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Thank you for getting back to me.
I think that sounds like a good idea. I would say that trying a 15% or 20% gel would probably perform better. Otherwise, make sure that the protein is not run off the gel (stop it well before the dye front reached...

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Thank you for getting back to me with this information and sorry for the delay in my reply.
The reason I asked about the ladder is because the Metallothionein protein is very small and it is therefore important to make sure that the lower end of t...

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Thank you for your reply.
I'd like to clarify a few details if you wouldn't mind in order to understand the problems encountered with the Anti-Metallothionein antibody [UC1MT] (ab12228) more fully.
1. You mentioned that with 10 ug loaded ther...

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