Anti-Metabotropic Glutamate Receptor 7 抗体 (ab53705)

製品の概要

  • 製品名Anti-Metabotropic Glutamate Receptor 7 antibody
    Metabotropic Glutamate Receptor 7 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Metabotropic Glutamate Receptor 7
  • 特異性ab53705 detects endogenous levels of total Metabotropic Glutamate Receptor 7 protein
  • アプリケーション適用あり: IP, WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from human Metabotropic Glutamate Receptor 7.

  • ポジティブ・コントロール
    • Human brain tissue and HuvEc cell extracts.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab53705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

ターゲット情報

Anti-Metabotropic Glutamate Receptor 7 antibody 画像

  • Metabotropic Glutamate Receptor 7 was immunoprecipitated using 0.5mg Rat Brain tissue extract, 5µg of Rabbit polyclonal to Metabotropic Glutamate Receptor 7 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Rat Brain tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab53705.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 110kDa; Metabotropic Glutamate Receptor 7

  • ab53705 at 1/50 dilution staining Metabotropic Glutamate Receptor 7 in human brain by Immunohistochemsitry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.ab53705 at 1/50 dilution staining Metabotropic Glutamate Receptor 7 in human brain by Immunohistochemsitry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-Metabotropic Glutamate Receptor 7 antibody (ab53705) at 1/500 dilution

    Lane 1 : HuvEc cell extract
    Lane 2 : HuvEc cell extract with immunising peptide


    Predicted band size : 102 kDa
    Observed band size : 102 kDa

Anti-Metabotropic Glutamate Receptor 7 antibody (ab53705) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Commercial Peroxidase blocking solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Commercial High pH Buffer
Sample Human Tissue sections (Tendon)
Specification Tendon
Permeabilization No
Fixative Formaldehyde
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Dr. Sarah Franklin

Verified customer

投稿 Aug 14 2014

Thank you for contacting us.
The immunogen sequence is from C-terminal end.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"