Anti-Metabotropic Glutamate Receptor 5 (phospho S996) 抗体 (ab16653)

製品の概要

  • 製品名
    Anti-Metabotropic Glutamate Receptor 5 (phospho S996) antibody
    Metabotropic Glutamate Receptor 5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Metabotropic Glutamate Receptor 5 (phospho S996)
  • 特異性
    ab16653 is raised against an immunizing peptide that recognises mGluR5 (phospho S996). This serine residue is numbered as 996 rather than as 1016 (which is how it is numbered on the database for rat mGluR5; Swiss Prot ID P31424), as the first 20aa of the protein are part of a cleaved signal peptide sequence. ab16653 specifically detects mGluR5 (phospho S996) protein expression in mouse hippocampal lysate at the expected band size (~130kDa). However, another stronger band is observed at >200kDa with ab16653 as well as with a positive control Rabbit polyclonal mGluR5 antibody. We are unsure of the identity of this larger band. However, both the 130kDa and 200kDa bands are blocked by preincubation with the immunizing peptide for ab16653.
  • アプリケーション
    適用あり: WB, IHC-FoFr, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Chicken, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1000 - 1100 of Rat Metabotropic Glutamate Receptor 5, phosphorylated at S994.

    (Peptide available as ab17573.)

  • ポジティブ・コントロール
    • mouse hippocampus lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 130 kDa).
IHC-FoFr 1/300.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
  • 配列類似性
    Belongs to the G-protein coupled receptor 3 family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Glutamate receptor metabotropic 5 antibody
    • GPRC1E antibody
    • Grm5 antibody
    • GRM5_HUMAN antibody
    • Metabotropic glutamate receptor 5 antibody
    • Metabotropic glutamate receptor 5 variant F antibody
    • Metabotropic glutamate receptor 5 variant G antibody
    • Metabotropic glutamate receptor 5 variant H antibody
    • mGlu5 antibody
    • mGluR5 antibody
    • PPP1R86 antibody
    • Protein phosphatase 1 regulatory subunit 86 antibody
    see all

画像

  • ICC/IF image of ab16653 stained rat PC12 cells. The cells were methanol fixed (5 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab16653, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • IHC-FoFr image of Metaobotropic Glutamate Receptor 5 (pS996) staining on rat brain sections using ab16653 (1:300). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.

    See Abreview

参考文献

ab16653 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

投稿 Jul 06 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain sections)
Specification
Brain sections
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Username

Dr. Sophie Pezet

Verified customer

投稿 Feb 16 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (Mouse cortex)
Loading amount
15 µg
Specification
Mouse cortex
Gel Running Conditions
Reduced Denaturing (4-12 % Bis-Tris)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jun 25 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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