Anti-Metabotropic Glutamate Receptor 4 抗体 (ab53088)

製品の概要

  • 製品名Anti-Metabotropic Glutamate Receptor 4 antibody
    Metabotropic Glutamate Receptor 4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Metabotropic Glutamate Receptor 4
  • アプリケーション適用あり: ICC/IF, IHC-P, IHC-Fr, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Cat, Human
  • 免疫原

    Synthetic peptide derived from human Metabotropic Glutamate Receptor 4 (C-terminal)

  • ポジティブ・コントロール
    • 293 cell extracts.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/200.
WB 1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
ELISA 1/5000.

ターゲット情報

Anti-Metabotropic Glutamate Receptor 4 antibody 画像

  • All lanes : Anti-Metabotropic Glutamate Receptor 4 antibody (ab53088) at 1/500 dilution

    Lane 1 : 293 cell extracts, treated with Forskolin (40nM, 30mins)
    Lane 2 : 293 cell extracts, treated with Forskolin (40nM, 30mins) with peptide


    Predicted band size : 102 kDa
    Observed band size : 102 kDa
  • Ab53088 staining Human cerebellum. Staining is localised to the cell membrane.
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ab53088 staining the Metabotropic Glutamate Receptor 4 in Rat heart tissue sections by IHC-Fr (frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 1% Triton and blocked with 3% serum for 2 hours at 20°C. Samples were incubated with primary antibody (1/200) for 12 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

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  • ICC/IF image of ab53088 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53088, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Metabotropic Glutamate Receptor 4 antibody (ab53088) 使用論文

This product has been referenced in:
  • Chruscicka B  et al. Tetracycline-based system for controlled inducible expression of group III metabotropic glutamate receptors. J Biomol Screen 20:350-8 (2015). Read more (PubMed: 25394730) »
  • Wang H  et al. Spinal metabotropic glutamate receptor 4 is involved in neuropathic pain. Neuroreport 22:244-8 (2011). Read more (PubMed: 21358553) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Cat Tissue sections (brainstem)
Antigen retrieval step None
Permeabilization Yes - 0.3 Triton
Specification brainstem
Blocking step Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative Paraformaldehyde
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投稿 Sep 10 2015

Application Immunohistochemistry (Frozen sections)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Sample Cat Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.1% triton
Fixative Paraformaldehyde
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投稿 Oct 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step None
Sample Chicken Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.1% triton
Fixative Paraformaldehyde
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投稿 Sep 26 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample Rat Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.3% triton
Fixative Paraformaldehyde
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投稿 Sep 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Sample Mouse Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.1% triton
Fixative Paraformaldehyde
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投稿 Sep 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample Chicken Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.2% triton
Fixative Paraformaldehyde
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投稿 Sep 20 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample Mouse Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.3% triton
Fixative Paraformaldehyde
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Verified customer

投稿 Sep 19 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample Rat Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.1% triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 19 2013

Thank you for contacting us and your interest in our products.

I am sorry for the delay in getting back to you but I can now share with you the protocol used to obtain the Western blotting image presented on the datasheet of Anti-Metabotropic...

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Thank you for contacting us.
The immunogen sequence is from C-terminal end.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"