Anti-Metabotropic Glutamate Receptor 2+3 抗体 (ab6438)

製品の概要

  • 製品名
    Anti-Metabotropic Glutamate Receptor 2+3 antibody
  • 製品の詳細
    Rabbit polyclonal to Metabotropic Glutamate Receptor 2+3
  • アプリケーション
    適用あり: IHC-P, IHC-Fr, WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Cat
    交差が予測される動物種: Mammal
  • 免疫原

    This antibody was raised against a synthetic peptide corresponding to the 13 c-terminus amino acids of mGluR2/3 with an N-appended lysine coupled to thyroglobulin using glutaraldehyde.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Tris - glycine, 0.1% sodium azide
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6438 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/500.
WB 1/1000. Detects a band of approximately 100, 200 kDa.
IP 1/100.

ターゲット情報

  • 関連性
    L glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. The activity of GRM2 and GRM3 receptors are mediated by a G-protein that inhibits adenylate cyclase activity.
  • 細胞内局在
    Integral membrane protein.
  • 参照データベース
  • 別名
    • GLUR2 antibody
    • GLUR3 antibody
    • Glutamate receptor homolog antibody
    • Glutamate receptor metabotropic 2 antibody
    • Glutamate receptor metabotropic 3 antibody
    • GPRC1B antibody
    • GPRC1C antibody
    • GRM2 antibody
    • GRM3 antibody
    • Metabotropic glutamate receptor 2 antibody
    • Metabotropic glutamate receptor 3 antibody
    • mGlu2 antibody
    • mGlu3 antibody
    • MGLUR2 antibody
    • MGLUR3 antibody
    see all

画像

  • ab6438 staining the Metabotropic Glutamate Receptor 2+3 in Chicken brain tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocked with 5% serum for 1 hour 30 minutes at 20°C. The sample was incubated with primary antibody (1/100) at 4°C for 8 hours. Ab6720 (1/400) was used as secondary antibody.

    See Abreview

  • ab6438 staining Metabotropic Glutamate Receptor 2+3 in rat retinal tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde, blocked using 10% serum for 20 minutes at 24°C, then incubated with ab6438 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 (green) conjugated donkey anti-rabbit polyclonal, used at a 1/1000 dilution. Counterstained with Hoechst 33258 (blue).

    GCL - ganglion cell layer
    IPL - inner plexiform layer
    INL - inner nuclear layer
    OPL - outer plexiform layer
    ONL - outer nuclear layer

    See Abreview

参考文献

This product has been referenced in:
  • Chen YW  et al. Activation of mGluR2/3 underlies the effects of N-acetylcystein on amygdala-associated autism-like phenotypes in a valproate-induced rat model of autism. Front Behav Neurosci 8:219 (2014). WB ; Rat . Read more (PubMed: 24987341) »
  • Tang ZQ  et al. Activation of synaptic group II metabotropic glutamate receptors induces long-term depression at GABAergic synapses in CNS neurons. J Neurosci 33:15964-77 (2013). Read more (PubMed: 24089501) »

See all 3 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Sample
Mouse Cultured Cells (brain)
Specification
brain
Permeabilization
Yes - 0.3% triton
Fixative
Methanol
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Abcam user community

Verified customer

投稿 Sep 18 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
(agent) for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Chicken Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde
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Abcam user community

Verified customer

投稿 Sep 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Chicken Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Mouse Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Rat Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.3 triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Sample
Mouse Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Sample
Rat Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 16 2013

Thank you for your phone call.
Unfortunately, the blocking peptides for these antibodies are not available for sale through Abcam. You may be able to find existing peptides on biocompare.com, or have them synthesized using the following sequences:...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Retina)
Specification
Retina
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Sep 13 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (cerebellum)
Specification
cerebellum
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Permeabilization
Yes - triton X-100
Blocking step
Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

投稿 Aug 12 2011

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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