Anti-Metabotropic Glutamate Receptor 2+3 抗体 (ab6438)
製品の概要
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製品名Anti-Metabotropic Glutamate Receptor 2+3 antibody
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製品の詳細Rabbit polyclonal to Metabotropic Glutamate Receptor 2+3
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由来種Rabbit
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アプリケーション適用あり: IHC-P, IHC-Fr, WB, IPmore details
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種交差性交差種: Mouse, Rat, Chicken, Cat
交差が予測される動物種: Mammal
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免疫原
This antibody was raised against a synthetic peptide corresponding to the 13 c-terminus amino acids of mGluR2/3 with an N-appended lysine coupled to thyroglobulin using glutaraldehyde.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーTris - glycine, 0.1% sodium azide
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Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab6438 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | 1/500. | |
| WB | 1/1000. Detects a band of approximately 100, 200 kDa. | |
| IP | 1/100. |
ターゲット情報
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関連性L glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. The activity of GRM2 and GRM3 receptors are mediated by a G-protein that inhibits adenylate cyclase activity.
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細胞内局在Integral membrane protein.
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参照データベース
- Entrez Gene: 108068 Mouse
- Entrez Gene: 108069 Mouse
- Entrez Gene: 24415 Rat
- Entrez Gene: 24416 Rat
- SwissProt: Q9QYS2 Mouse
- SwissProt: P31421 Rat
- SwissProt: P31422 Rat
- Unigene: 41715 Rat
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別名
- GLUR2 antibody
- GLUR3 antibody
- Glutamate receptor homolog antibody
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画像
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Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 2+3 antibody (ab6438)This image is courtesy of an anonymous Abreviewab6438 staining the Metabotropic Glutamate Receptor 2+3 in Chicken brain tissue sections by IHC-Fr (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocked with 5% serum for 1 hour 30 minutes at 20°C. The sample was incubated with primary antibody (1/100) at 4°C for 8 hours. Ab6720 (1/400) was used as secondary antibody. -
Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 2+3 antibody (ab6438)Image courtesy of an anonymous Abreview.ab6438 staining Metabotropic Glutamate Receptor 2+3 in rat retinal tissue by Immunohistochemistry (Frozen sections).
Tissue was fixed in paraformaldehyde, blocked using 10% serum for 20 minutes at 24°C, then incubated with ab6438 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 488 (green) conjugated donkey anti-rabbit polyclonal, used at a 1/1000 dilution. Counterstained with Hoechst 33258 (blue).
GCL - ganglion cell layerIPL - inner plexiform layer
INL - inner nuclear layerOPL - outer plexiform layer
ONL - outer nuclear layer
参考文献
This product has been referenced in:
- Goniotaki D et al. Inhibition of group-I metabotropic glutamate receptors protects against prion toxicity. PLoS Pathog 13:e1006733 (2017). Read more (PubMed: 29176838) »
- Chen YW et al. Activation of mGluR2/3 underlies the effects of N-acetylcystein on amygdala-associated autism-like phenotypes in a valproate-induced rat model of autism. Front Behav Neurosci 8:219 (2014). WB ; Rat . Read more (PubMed: 24987341) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"