Anti-Metabotropic Glutamate Receptor 2 抗体 [mG2Na-s] (ab15672)

製品の概要

  • 製品名
    Anti-Metabotropic Glutamate Receptor 2 antibody [mG2Na-s]
    Metabotropic Glutamate Receptor 2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [mG2Na-s] to Metabotropic Glutamate Receptor 2
  • 特異性
    This antibody recognizes the metabotrophic glutamate receptor 2, but not metabotrophic glutamate receptor 3.
  • アプリケーション
    適用あり: IHC-Fr, WB, ICC/IF, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    Fusion protein corresponding to Metabotropic Glutamate Receptor 2. Fusion protein contains a 47 amino acid sequence of the N terminal of the Metabotrophic Glutamate Receptor 2

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: PBS, pH 7.4 sterile filtered
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    mG2Na-s
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab15672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use a concentration of 1 µg/ml. See reference Neki et al, Neurosci Letts.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 104 kDa). See reference Neki et al, Neurosci Letts.
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. May mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.
  • 組織特異性
    Widely expressed in different regions of the adult brain as well as in fetal brain.
  • 配列類似性
    Belongs to the G-protein coupled receptor 3 family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AMPA selective glutamate receptor 2 antibody
    • GLUR2 antibody
    • GLURB antibody
    • Glutamate metabotropic receptor 2 antibody
    • Glutamate receptor homolog antibody
    • Glutamate receptor metabotropic 2 antibody
    • GPRC1B antibody
    • GRM2 antibody
    • GRM2_HUMAN antibody
    • Metabotropic glutamate receptor 2 antibody
    • mGlu2 antibody
    • mGluR2 antibody
    • OTTHUMP00000210984 antibody
    • OTTHUMP00000210986 antibody
    see all

画像

  • Anti-Metabotropic Glutamate Receptor 2 antibody [mG2Na-s] (ab15672) at 1 µg/ml + Brain (Rat) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 104 kDa
    Observed band size : 99 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • Immunohistochemical analysis of mouse cortical neurons, staining Metabotropic Glutamate Receptor 2 with ab15672. Donkey anti-mouse 6-nm colloidal gold particles were used to detect staining. Images were visualized by electron microscopy.

参考文献

This product has been referenced in:
  • Cao DY  et al. EXPRESS: Histone hyperacetylation modulates spinal type II metabotropic glutamate receptor alleviating stress-induced visceral hypersensitivity in female rats. Mol Pain 12:N/A (2016). WB . Read more (PubMed: 27385724) »
  • Moreno JL  et al. Allosteric signaling through an mGlu2 and 5-HT2A heteromeric receptor complex and its potential contribution to schizophrenia. Sci Signal 9:ra5 (2016). Read more (PubMed: 26758213) »

See all 13 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Human Tissue lysate - whole (Human brain lysate)
Gel Running Conditions
Reduced Denaturing (Samples heated 5 min at 37 degrees, 4%-15% gel)
Loading amount
10 µg
Specification
Human brain lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Oct 11 2016

You are correct, this antibody recognizes a portion of the N-terminus of mGluR2.

I am glad to read that most of the antibodies you ordered are giving good results. For the two that are not, could you please send some details of the samples and protocols and results? In particular, we would like to know the species, the time in PFA,...

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Gracias por tu respuesta.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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