Anti-Metabotropic Glutamate Receptor 1a 抗体 (ab51314)

製品の概要

  • 製品名Anti-Metabotropic Glutamate Receptor 1a antibody
    Metabotropic Glutamate Receptor 1a 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Metabotropic Glutamate Receptor 1a
  • 特異性IHC-P results for Mouse have been shown to be negative for this antibody. Although, there is significant homology with Mouse, it is not predicted species.
  • アプリケーション適用あり: ICC/IF, WB, IHC (PFA fixed)more details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Cynomolgus Monkey
  • 免疫原

    Synthetic peptide:

    SSVPSSPVSESVL

    , corresponding to amino acids 1159-1171 of Rat Metabotropic Glutamate Receptor 1a.

  • ポジティブ・コントロール
    • Rat brain (singulate cortex) Human brain lysate Rat cerebellar lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab51314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
WB
IHC (PFA fixed)
  • 追加情報ICC/IF: 1/250.
    Immunohistochemistry (PFA fixed): 1/250.
    WB: 1/1000. Predicted molecular weight: 133 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum.
    • 組織特異性Detected in brain.
    • 関連疾患Spinocerebellar ataxia, autosomal recessive, 13
    • 配列類似性Belongs to the G-protein coupled receptor 3 family.
    • 細胞内局在Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Glutamate receptor metabotropic 1 antibody
      • GPRC1A antibody
      • GRM1 alpha antibody
      • GRM1 antibody
      • GRM1_HUMAN antibody
      • Metabotropic glutamate receptor 1 antibody
      • MGLU1 antibody
      • mGluR1 antibody
      • SCAR13 antibody
      see all

    Anti-Metabotropic Glutamate Receptor 1a antibody 画像

    • Metabotropic Glutamate Receptor 1a staining in rat brain (singulate cortex) using ab51314 at 1/100 dilution. Metabotropic Glutamate Receptor 1a detection was done using anti-rabbit Cy3 conjugated antibodies (red color). DAPI was used to counterstain cell nuclei (blue color).
    • Anti-Metabotropic Glutamate Receptor 1a antibody (ab51314) at 1/1000 dilution + Human brain lysate at 20 µg

      Secondary
      AP-conjugated secondary at 1/5000 dilution

      Predicted band size : 133 kDa
      Observed band size : 105 kDa (why is the actual band size different from the predicted?)
    • All lanes : Anti-Metabotropic Glutamate Receptor 1a antibody (ab51314)

      Lane 1 : Rat cerebellar membranes
      Lane 2 : Rat cerebellar membranes + DTT


      Predicted band size : 133 kDa
      Observed band size : 133 kDa

    Anti-Metabotropic Glutamate Receptor 1a antibody (ab51314) 使用論文

    This product has been referenced in:
    • Romano C  et al. Distribution of metabotropic glutamate receptor mGluR5 immunoreactivity in rat brain. J Comp Neurol 355:455-69 (1995). Read more (PubMed: 7636025) »

    See 1 Publication for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Ferret Tissue lysate - whole (Parietal cortex in Brain)
    Gel Running Conditions Reduced Denaturing (Nupage 3-8% Tris-acetate gel)
    Loading amount 40 µg
    Specification Parietal cortex in Brain
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Username

    Francis Djankpa

    Verified customer

    投稿 Jan 07 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"