Anti-Metabotropic Glutamate Receptor 1 抗体 (ab82211)

製品の概要

  • 製品名Anti-Metabotropic Glutamate Receptor 1 antibody
    Metabotropic Glutamate Receptor 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Metabotropic Glutamate Receptor 1
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, IHC-Fr, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Human
  • 免疫原

    Synthetic peptide:

    SSVPSSPVSESVL

    , corresponding to amino acids 1159-1171 of Human Metabotropic Glutamate Receptor 1

  • ポジティブ・コントロール
    • Human brain lysate, rat dorsal root ganglion, and rat brain (cingulate cortex).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 133 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/500.
IHC-Fr 1/200.
IHC-FoFr Use at an assay dependent concentration.

ターゲット情報

Anti-Metabotropic Glutamate Receptor 1 antibody 画像

  • Anti-Metabotropic Glutamate Receptor 1 antibody (ab82211) at 1/1000 dilution + human brain lysate at 20 µg

    Secondary
    AP-conjugated secondary at 1/5000 dilution

    Predicted band size : 133 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence labeling of Metabotropic Glutamate Receptor 1 in rat brain (cingulate cortex). Using ab82211 (red color). DAPI was used to counter-stain cell nuclei (blue color). Working dilution is 1/100-1/500.
  • Rat dorsal root ganglion. Tissue was fixed in 4% paraformaldehyde and cut into 10 micron sections. ab82211 was used at a dilution of 1/200 and secondary antibody at a dilution of 1/5000.
  • ab82211 staining Metabotropic Glutamate Receptor 1 in rat cerebellum tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde, permeabilized using Triton X-100, blocked with 7% serum for 3 hours at 25°C, then incubated with ab82211 at a 1/200 dilution for 10 hours at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.

    See Abreview

Anti-Metabotropic Glutamate Receptor 1 antibody (ab82211) 使用論文

This product has been referenced in:
  • Valiya Veettil M  et al. Glutamate secretion and metabotropic glutamate receptor 1 expression during Kaposi's sarcoma-associated herpesvirus infection promotes cell proliferation. PLoS Pathog 10:e1004389 (2014). Human . Read more (PubMed: 25299066) »
  • Sylantyev S  et al. Spike-driven glutamate electrodiffusion triggers synaptic potentiation via a homer-dependent mGluR-NMDAR link. Neuron 77:528-41 (2013). IHC-P . Read more (PubMed: 23395378) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step (agent) for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Sample Cat Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.3% triton
Fixative Paraformaldehyde
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Abcam user community

Verified customer

投稿 Oct 07 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample Chicken Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.3% triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample Rat Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.1% triton
Fixative Paraformaldehyde
Username

Dr. Yuhua Liu

Verified customer

投稿 Sep 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample Mouse Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.1% triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample Rat Cell (brain)
Specification brain
Permeabilization Yes - 0.3% triton
Fixative Methanol
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Abcam user community

Verified customer

投稿 Sep 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample Chicken Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.1% trition
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample Mouse Tissue sections (brainstem)
Specification brainstem
Permeabilization Yes - 0.3% triton
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample Rat Tissue sections (brainstem)
Specification brainstem
Permeabilization No
Fixative Paraformaldehyde
Username

Wei Shi

Verified customer

投稿 Jul 16 2013

I performed a search of some other anti - metabotropic glutamate receptor 1 antibodies, and it seems the glutamate receptor can run between ˜110 - 130 kDa depending on the post-translational modification in the tested samples. Thus, the 110 kDa band th...

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