Anti-Met Enkephalin 抗体 (ab22620)


  • 製品名Anti-Met Enkephalin antibody
  • 製品の詳細
    Rabbit polyclonal to Met Enkephalin
  • アプリケーション適用あり: ICC/IF, IHC-P, IHC-Rmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Met Enkephalin conjugated to Keyhole Limpet Haemocyanin (KLH) (Glutaraldehyde). The conjugate was dialysed to remove excess conjugating agent prior to immunisation.

  • ポジティブ・コントロール
    • Typical test tissues included rat and human brain, rat spinal cord, pig duodenum, guinea pig ileum (enteric nervous system) and human phaeochromocytoma (adrenomedullary tumour)



Our Abpromise guarantee covers the use of ab22620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/1000.
IHC-P Use at an assay dependent concentration.
IHC-R 1/500 - 1/4000. (Using overnight incubation and ABC/PAP procedure). Dilutions up to 1/10,000 have been achieved under optimised conditions. 1/200-1/4000, using overnight incubation and indirect immunofluorescence procedure. All standard crosslinking agents may be used, but formaldehyde based solutions are recommended. Frozen sections and Vibratome slices are recommended for tracttracing.


  • 機能Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.
  • 配列類似性Belongs to the opioid neuropeptide precursor family.
  • 翻訳後修飾The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Leu-enkephalin antibody
    • Met-enkephalin antibody
    • Met-enkephalin-Arg-Gly-Leu antibody
    • Met-enkephalin-Arg-Phe antibody
    • Methionine enkephalin antibody
    • OGF antibody
    • Opioid growth factor antibody
    • PENK antibody
    • PENK(114-133) antibody
    • PENK(143-183) antibody
    • PENK(237-258) antibody
    • PENK_HUMAN antibody
    • PPA antibody
    • Proenkephalin A antibody
    • Proenkephalin antibody
    • Proenkephalin-A antibody
    • Synenkephalin antibody
    see all

Anti-Met Enkephalin antibody 画像

  • ICC/IF image of ab22620 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22620, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab22620 (4µg/ml) staining met enkephalin in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining of seminal vesicles.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-Met Enkephalin antibody (ab22620) 使用論文

ab22620 has not yet been referenced specifically in any publications.

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I can confirm that ab22620 was tested and is guaranteed for human samples.

If your customer would like to use this antibody with another species, I will be happy to issue a testing discount.

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