Anti-Mesp1 抗体 (ab77013)

製品の概要

  • 製品名Anti-Mesp1 antibody
    Mesp1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to Mesp1
  • アプリケーション適用あり: ELISA, WB, ICC/IF, ICCmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-63 of Human Mesp1

  • ポジティブ・コントロール
    • FHs 173 WE cell lysate; Recombinant tagged full length human Mesp1.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab77013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 29 kDa.
ICC/IF Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

ターゲット情報

  • 機能Transcription factor. Plays a role in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. Defines the rostrocaudal patterning of the somites by participating in distinct Notch pathways.
  • 配列類似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • bHLHc5 antibody
    • Class C basic helix-loop-helix protein 5 antibody
    • Mesoderm posterior 1 antibody
    • Mesoderm posterior 1 homolog (mouse) antibody
    • Mesoderm posterior 1 homolog antibody
    • Mesoderm posterior protein 1 antibody
    • MESP 1 antibody
    • Mesp1 antibody
    • MESP1_HUMAN antibody
    • MGC10676 antibody
    • MGC159208 antibody
    • MGC159210 antibody
    • OTTMUSP00000030151 antibody
    see all

Anti-Mesp1 antibody 画像

  • Anti-Mesp1 antibody (ab77013) at 1 µg/ml + FHs 173 WE cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 29 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

Anti-Mesp1 antibody (ab77013) 使用論文

ab77013 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%
Sample Mouse Cell (E7.5 embryo)
Specification E7.5 embryo
Permeabilization Yes - 0.1% TritonX100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Oct 24 2014

Many thanks for contacting us last week in regards to antibodies raised against Mesp1.

As discussed over the phone, the anti-Mesp1 antibody (ab77013) has as yet not been used in ICC. This does not mean that it would not work, simply that we ha...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"