製品の概要

  • 製品名Anti-MERTK antibody [Y323]
    MERTK 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y323] to MERTK
  • アプリケーション適用あり: Blocking, WB, IP, IHC-Pmore details
    適用なし: Flow Cyt or ICC/IF
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MERTK aa 1-100 (N terminal).

  • ポジティブ・コントロール
    • WB: HEK293 and K562 cell lysate. IHC-P: Human tonsil and lymphoma tissues. IP: HEK293 cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:

    Anti-MERTK antibody (HRP) [Y323] (ab197643)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Blocking Use at an assay dependent concentration. PubMed: 19204785
WB 1/2000. Detects a band of approximately 180 kDa.

For unpurified use at 1/500.

IP 1/20.

For unpurified use at 1/50.

IHC-P 1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

  • 追加情報Is unsuitable for Flow Cyt or ICC/IF.
  • ターゲット情報

    • 機能In case of filovirus infection, seems to function as a cell entry factor.
    • 組織特異性Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines.
    • 関連疾患Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
    • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
      Contains 2 fibronectin type-III domains.
      Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 protein kinase domain.
    • 細胞内局在Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • c MER antibody
      • c mer proto oncogene tyrosine kinase antibody
      • c-mer antibody
      • cMER antibody
      • cmer protooncogene tyrosine kinase antibody
      • Eyk antibody
      • MER antibody
      • MER receptor tyrosine kinase antibody
      • MERK antibody
      • MERPEN antibody
      • Mertk antibody
      • MERTK c-mer proto-oncogene tyrosine kinase antibody
      • MERTK_HUMAN antibody
      • MGC133349 antibody
      • nmf12 antibody
      • Nyk antibody
      • Proto oncogene tyrosine protein kinase MER antibody
      • Proto oncogene tyrosine protein kinase MER precursor antibody
      • Proto-oncogene c-Mer antibody
      • Receptor tyrosine kinase MerTK antibody
      • RP38 antibody
      • STK kinase antibody
      • Tyrosine-protein kinase Mer antibody
      see all

    Anti-MERTK antibody [Y323] 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma tissue labelling MERTK with unpurified ab52968 at a 1/50 dilution.

    • All lanes : Anti-MERTK antibody [Y323] (ab52968) at 1/2000 dilution (purified)

      Lane 1 : HEK293 cell lysate
      Lane 2 : K562 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG at 1/1000 dilution

      Observed band size : 140-180 kDa (why is the actual band size different from the predicted?)

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-MERTK antibody [Y323] (ab52968) at 1/1000 dilution (unpurified) + HEK293 cell lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling MERTK with purified ab52968 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • ab52968 (purified) at 1/20 immunoprecipitating MERTK in HEK293 cell lysate (Lane 1). Lane 2 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    Anti-MERTK antibody [Y323] (ab52968) 使用論文

    This product has been referenced in:
    • Petta S  et al. MERTK rs4374383 polymorphism affects the severity of fibrosis in non-alcoholic fatty liver disease. J Hepatol 64:682-90 (2016). WB, IF, IHC ; Human . Read more (PubMed: 26596542) »
    • Minson KA  et al. The MERTK/FLT3 inhibitor MRX-2843 overcomes resistance-conferring FLT3 mutations in acute myeloid leukemia. JCI Insight 1:e85630 (2016). WB ; Human . Read more (PubMed: 27158668) »

    See all 19 Publications for this product

    Product Wall

    Unfortunately, in few cases we cannot find the connection between the antibody used in a publication and the antibody used in our catalog, especially as we are trying to link this kind of information to the datasheets.
    However, as we have only two...

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    Thank you for contacting us and your interest in our products.

    I can now confirm that the concentration of the anti-MERTK antibody [Y323] (ab52968), of the lot number GR54920-5 is 0.1180 mg/ml.

    I hope this information has been of he...

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    Thank you for contacting us and sorry for the delay of our reply.
    Please find below the reply I just received from the source of the anti-MERTK antibody [Y323], ab52968:
    > "Sorry for the delay in response. As stated previously, the anti-MERTK...

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    I just wanted to let you know that we are still waiting for a reply from the originator of this product. I have sent a reminder and I hope we will receive the details about the IHC image you requested in the very near future.
    I am very sorry ...

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    Thank you for contacting us. I apologise for the waiting.
    Anti-MERTK antibody [Y323] (ab52968) displayed clear membranous and some cytoplasmic IHC staining of human lymphoma tissue (see larger image attached).
    The Human Protein Atlas states “...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (cell lines)
    Loading amount 20 µg
    Specification cell lines
    Gel Running Conditions Reduced Denaturing (4-15% Tris-glycine gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Mr. Nir Drayman

    Verified customer

    投稿 Mar 05 2012

    Thank you for contacting Abcam. The antibody, ab52968, anti-MERTK antibody [Y323], is covered under our Abpromise, which means that if the antibody does not work as stated on the data sheet and if our support specialist cannot help resolve the issue, t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"