製品の概要

  • 製品名Anti-MERTK antibody
    MERTK 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MERTK
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 900 to the C-terminus of Mouse MERTK.

    (Peptide available as ab106208.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: NIH 3T3; RAW 264.7.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab95925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 145 kDa (predicted molecular weight: 110 kDa).
ICC/IF Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能In case of filovirus infection, seems to function as a cell entry factor.
  • 組織特異性Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines.
  • 関連疾患Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
  • 配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • c MER antibody
    • c mer proto oncogene tyrosine kinase antibody
    • c-mer antibody
    • cMER antibody
    • cmer protooncogene tyrosine kinase antibody
    • Eyk antibody
    • MER antibody
    • MER receptor tyrosine kinase antibody
    • MERK antibody
    • MERPEN antibody
    • Mertk antibody
    • MERTK c-mer proto-oncogene tyrosine kinase antibody
    • MERTK_HUMAN antibody
    • MGC133349 antibody
    • nmf12 antibody
    • Nyk antibody
    • Proto oncogene tyrosine protein kinase MER antibody
    • Proto oncogene tyrosine protein kinase MER precursor antibody
    • Proto-oncogene c-Mer antibody
    • Receptor tyrosine kinase MerTK antibody
    • RP38 antibody
    • STK kinase antibody
    • Tyrosine-protein kinase Mer antibody
    see all

Anti-MERTK antibody 画像

  • All lanes : Anti-MERTK antibody (ab95925) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 110 kDa
    Observed band size : 145 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
    MERTK protein contains a number of potential glycosylation sites (SwissProt), which may explain its migration at a higher molecular weight than predicted.
  • ICC/IF image of ab95925 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab95925 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MERTK antibody (ab95925) 使用論文

ab95925 has not yet been referenced specifically in any publications.

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