This antibody was developed to improve detection of melphalan-DNA adducts through its ability to recognise the stabilised adduct. This antibodiy can be used for quantification of melphalan adducts in purified DNA by ELISA and for immunofluorescent detection of melphalan-DNA adducts in single cells.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent dilution.
Mephalan hydrochloride is a chemotherapy drug belonging to the class of nitrogen mustard alkylating agents.
This product has been referenced in:
Rothbarth J et al. Immunohistochemical detection of melphalan-DNA adducts in colon cancer cells in vitro and human colorectal liver tumours in vivo. Biochem Pharmacol67:1771-8 (2004).
Read more (PubMed: 15081876) »
Frank AJ & Tilby MJ Quantification of DNA adducts in individual cells by immunofluorescence: effects of variation in DNA conformation. Exp Cell Res283:127-34 (2003).
Read more (PubMed: 12581733) »