製品の概要

  • 製品名Anti-MEPCE antibody
    MEPCE 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to MEPCE
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Horse, Dog, Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    KQQRKFQYGNYCK

    , corresponding to internal sequence amino acids 408-420 of Human MEPCE (NP_062552.2).

  • ポジティブ・コントロール
    • Rat testis lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab77329 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 関連性MEPCE belongs to the methyltransferase superfamily and contains one BIN3 domain. It is an S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it. MEPCE is expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. It is weakly or not expressed in other tissues.
  • 参照データベース
  • 別名
    • 7SK snRNA methylphosphate capping enzyme antibody
    • BCDIN3 antibody
    • Bicoid interacting protein 3 homolog antibody
    • Bin3 bicoid interacting 3 antibody
    • Bin3 bicoid interacting 3 homolog (Drosophila) antibody
    • Bin3 bicoid interacting 3 homolog antibody
    • Bin3 homolog antibody
    • FLJ20257 antibody
    • MePCE antibody
    • Methylphosphate capping enzyme antibody
    see all

Anti-MEPCE antibody 画像

  • ab77329 (5µg/ml) staining of MEPCE in paraffin embedded Human Testis following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows staining of the spermatids.

  • Anti-MEPCE antibody (ab77329) at 1 µg/ml + rat testis lysate in RIPA buffer at 35 µg

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
    Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-MEPCE antibody (ab77329) 使用論文

ab77329 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"