Membrane Integrity WB 抗体 Cocktail (ab110414)
Key features and details
- Assay type: Quantitative
製品の概要
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製品名
Membrane Integrity WB Antibody Cocktail -
アッセイタイプ
Quantitative -
種交差性
交差種: Mouse, Rat, Cow, Human -
製品の概要
ab110414 (MS620) contains 5 mAbs against representative proteins for each of the primary structures of the mitochondrion:
Outer Membrane - Porin ab14734 (MSA03)
Intermembrane Space - Cytochrome c ab110325 (MSA06)
Inner Membrane - Complex Va ab110273 (MS502) and Complex III Core 1 ab110252 (MS303)
Matrix Space - Cyclophilin D ab110324 (MSA04)
This kit is useful in Western blotting to evaluate the structural integrity of isolated mitochondria. This kit is reactive against 39kDa human porin, 12kDa cytochrome c, 55kDa Complex Va, 49kDa Complex III Core 1, and 21kDa Cyclophilin D.
Cocktail Antibodies:
- Mouse monoclonal [20B12AF2] to VDAC1 / Porin - Mitochondrial Loading Control (ab14734):
Amount: 50µg
Working concentration: 2µg/ml - Mouse monoclonal [E11AE12BD4] to Cyclophilin 40 (ab110324):
Amount: 50µg
Working concentration: 2µg/ml - Mouse monoclonal [16D10AD9AH5] to Ubiquinol-Cytochrome C Reductase Core Protein I (ab110252):
Amount: 50µg
Working concentration: 2µg/ml - Mouse monoclonal [37BA11] to Cytochrome C (ab110325):
Amount: 5µg
Working concentration: 0.1µg/ml - Mouse monoclonal [7H10BD4F9] to ATP5A (ab110273):
Amount: 100µg
Working concentration: 4µg/ml
- Mouse monoclonal [20B12AF2] to VDAC1 / Porin - Mitochondrial Loading Control (ab14734):
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アプリケーション
適用あり: WBmore details
製品の特性
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保存方法
Store at -80°C. Please refer to protocols. -
内容 255 µg Cocktail of 5 Antibodies 1 x 255µg Rat liver mitochondria control 1 x 50µg -
研究分野
関連製品
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Positive Controls
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab110414の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 10.2 µg/ml. The antibody cocktail (2.37 mg/mL) should be diluted to a final working concentration of10.2 µg/mL for Western blotting.
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特記事項 |
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WB
Use a concentration of 10.2 µg/ml. The antibody cocktail (2.37 mg/mL) should be diluted to a final working concentration of10.2 µg/mL for Western blotting. |
画像
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All lanes : Membrane Integrity WB Antibody Cocktail (ab110414)
Lane 1 : Rat liver mitos prep #1
Lane 2 : Rat liver mitos prep #2
Lysates/proteins at 10 µg per lane. -
Sample: Mouse whole cell lysate (3T3 SA cell line)
Loading amount: 20µg
Gel Running conditions: reduced denaturating (4-12% gradient gel)
Blocking step: 30 min in 5% milk at room temperature
Dilution: 1/1000
Incubation time: Overnight at 4°C or 1 hour at room temperature
Secondary antibody: Goat anti-mouse horseradish peroxidase linked, 1/5000 dilution
Detection: ECL
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab110414 は 21 報の論文で使用されています。
- Zhang Z et al. Ruthenium 360 and mitoxantrone inhibit mitochondrial calcium uniporter channel to prevent liver steatosis induced by high-fat diet. Br J Pharmacol 179:2678-2696 (2022). PubMed: 34862596
- Tokarsky EJ et al. Mitochondrial Dysfunction Is a Driver of SP-2509 Drug Resistance in Ewing Sarcoma. Mol Cancer Res 20:1035-1046 (2022). PubMed: 35298000
- Zhang Z et al. Dietary calcium supplementation promotes the accumulation of intramuscular fat. J Anim Sci Biotechnol 12:94 (2021). PubMed: 34503581
- Zhang Z et al. Calcium supplementation relieves high-fat diet-induced liver steatosis by reducing energy metabolism and promoting lipolysis. J Nutr Biochem 94:108645 (2021). PubMed: 33838230
- Seitaj B et al. Transmembrane BAX Inhibitor-1 Motif Containing Protein 5 (TMBIM5) Sustains Mitochondrial Structure, Shape, and Function by Impacting the Mitochondrial Protein Synthesis Machinery. Cells 9:N/A (2020). PubMed: 32977469