製品の概要

  • 製品名
    Anti-Melatonin Receptor 1A antibody
    Melatonin Receptor 1A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Melatonin Receptor 1A
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Dog
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 296-345 (GLLNQNFRKE YRRIIVSLCT ARVFFVDSSN DVADRVKWKP SPLMTNNNVV) of Human Melatonin Receptor 1A (NP_005949).

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab116337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 39 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    High affinity receptor for melatonin. Likely to mediates the reproductive and circadian actions of melatonin. The activity of this receptor is mediated by pertussis toxin sensitive G proteins that inhibit adenylate cyclase activity.
  • 組織特異性
    Expressed in hypophyseal pars tuberalis and hypothalamic suprachiasmatic nuclei (SCN). Hippocampus.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Mel 1A R antibody
    • MEL-1A-R antibody
    • Mel1a melatonin receptor antibody
    • Mel1a receptor antibody
    • Melatonin receptor 1A antibody
    • Melatonin receptor type 1A antibody
    • MelR antibody
    • MR antibody
    • MT 1 antibody
    • MT1 antibody
    • MTNR 1A antibody
    • MTNR1A antibody
    • MTR1A_HUMAN antibody
    see all

画像

  • Anti-Melatonin Receptor 1A antibody (ab116337) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Predicted band size : 39 kDa

プロトコール

参考文献

This product has been referenced in:
  • Guo P  et al. Melatonin Improves Mitochondrial Function by Promoting MT1/SIRT1/PGC-1 Alpha-Dependent Mitochondrial Biogenesis in Cadmium-Induced Hepatotoxicity In Vitro. Toxicol Sci N/A:N/A (2014). Human . Read more (PubMed: 25159133) »

See 1 Publication for this product

レビューと Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0 12 min at 90 degrees celsius
Permeabilization
No
Specification
Pancreas
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jun 30 2017

Application
Western blot
Sample
Human Tissue lysate - whole (Abdominal endometriotic lesion)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
15 µg
Specification
Abdominal endometriotic lesion
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Mar 21 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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