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Our Abpromise guarantee covers the use of ab19306 in the following tested applications.
|WB||Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 53,85 kDa. Endogenous melanopsin is expressed at low levels making it difficult to detect. We recommend running a positive control and conducting experiments on samples overexpressing melanopsin protein.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 10 µg/ml.|
Immunofluorescence and immunocytochemistry staining of Melanopsin in rat retinal ganglion cells (RGCs) can be performed using ab19306.
By Western blot, this antibody detects ~53 and ~85 kDa proteins representing overexpressed unglycosylated and glycosylated rat melanopsin from HEK 293 cells.