Anti-Melanoma Inhibitory Activity 抗体 (ab110612)

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製品の概要

  • 製品名
    Anti-Melanoma Inhibitory Activity antibody
    Melanoma Inhibitory Activity 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Melanoma Inhibitory Activity
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: Flow Cyt, Indirect ELISA, Sandwich ELISA, WBmore details
  • 種交差性
    交差種: Human, Recombinant fragment
  • 免疫原

    Highly pure (>98%) recombinant Human Melanoma Inhibitory Activity

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab110612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/100.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 
Indirect ELISA Use a concentration of 0.5 - 2 µg/ml. (using 100 µl/well antibody solution) allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human Melanoma Inhibitory Activity.
Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Melanoma Inhibitory Activity (Biotin) (ab84235). Using ab84235 as the detection antibody and ab110612 as the capture antibody, the detection limit for recombinant Human Melanoma Inhibitory Activity is 0.2 - 0.4 ng/well, using 100 µl/well antibody solution.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 15 kDa. The detection limit for recombinant Human Melanoma Inhibitory Activity is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.

ターゲット情報

  • 機能
    Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
  • 組織特異性
    All malignant melanoma cell lines tested and infrequently in glioma cell lines.
  • 配列類似性
    Belongs to the MIA/OTOR family.
    Contains 1 SH3 domain.
  • 翻訳後修飾
    May possess two intramolecular disulfide bonds.
  • 細胞内局在
    Secreted.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Cartilage derived retinoic acid sensitive protein antibody
      • CD RAP antibody
      • CDRAP antibody
      • Melanoma derived growth regulatory protein precursor antibody
      • Melanoma inhibitory activity protein antibody
      • Melanoma-derived growth regulatory protein antibody
      • Mia antibody
      • MIA_HUMAN antibody
      see all

    画像

    • Flow Cytometry - Anti-Melanoma Inhibitory Activity antibody (ab110612)
      Flow Cytometry - Anti-Melanoma Inhibitory Activity antibody (ab110612)This image is courtesy of an anonymous Abreview

      ab110612 staining Melanoma Inhibitory Activity in Human melanoma cell line by Flow Cytometry. Cells were fixed with formaldehyde and permeabilized with saponine. The sample was incubated with the primary antibody (1/1000 in PBS) for 15 minutes at 20°C. ab97050, Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.

       

      See Abreview

    参考文献

    ab110612 has not yet been referenced specifically in any publications.

    ab110612 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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    Flow Cytometry abreview for Anti-Melanoma Inhibitory Activity antibody

     Good
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Fixation
    Formaldehyde
    Permeabilization
    Yes - Saponine
    Sample
    Human Cell (melanoma cell line)
    Specification
    melanoma cell line
    Gating Strategy
    cell line only
    Preparation
    Cell harvesting/tissue preparation method: EDTA (Versen) for harvesting cell
    Sample buffer: PBS
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    投稿 Jul 01 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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