製品の概要

  • 製品名Anti-Melanoma gp100 antibody
    Melanoma gp100 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Melanoma gp100
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human Melanoma gp100 aa 411-440 (internal sequence) conjugated to keyhole limpet haemocyanin. Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 411-440 of Human Melanoma gp100 (NP_008859.1).
    Database link: P40967

  • ポジティブ・コントロール
    • CEM cell line lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab116582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Predicted molecular weight: 70 kDa.

ターゲット情報

  • 機能Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
  • 組織特異性Preferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas. Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth.
  • 配列類似性Belongs to the PMEL/NMB family.
    Contains 1 PKD domain.
  • ドメインThe RPT domain is essential for the generation of the fibrillar matrix of melanosomes.
    The lumenal domain is necessary for correct processing and trafficking to melanosomes.
  • 翻訳後修飾A small amount of P1/P100 (major form) undergoes glycosylation to yield P2/P120 (minor form). P2 is cleaved by a furin-like proprotein convertase (PC) in a pH-dependent manner in a post-Golgi, prelysosomal compartment into two disulfide-linked subunits: a large lumenal subunit, M-alpha/ME20-S, and an integral membrane subunit, M-beta. Despite cleavage, only a small fraction of M-alpha is secreted, whereas most M-alpha and M-beta remain associated with each other intracellularly. M-alpha is further processed to M-alpha N and M-alpha C. M-alpha C further undergoes processing to yield M-alpha C1 and M-alpha C3 (M-alpha C2 in the case of PMEL17-is or PMEL17-ls). Formation of intralumenal fibrils in the melanosomes requires the formation of M-alpha that becomes incorporated into the fibrils. Stage II melanosomes harbor only Golgi-modified Pmel17 fragments that are derived from M-alpha and that bear sialylated O-linked oligosaccharides.
    N-glycosylated. O-glycosylated; contains sialic acid.
  • 細胞内局在Secreted and Endoplasmic reticulum membrane. Golgi apparatus. Melanosome. Endosome > multivesicular body. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes predominantly to intralumenal vesicles (ILVs) within multivesicular bodies. Associates with ILVs found within the lumen of premelanosomes and melanosomes and particularly in compartments that serve as precursors to the striated stage II premelanosomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 95 kDa melanocyte specific secreted glycoprotein antibody
    • 95 kDa melanocyte-specific secreted glycoprotein antibody
    • D12S53E antibody
    • gp100 antibody
    • M-beta antibody
    • ME20 antibody
    • ME20 M/ME20 S antibody
    • ME20-M antibody
    • ME20-S antibody
    • ME20M antibody
    • ME20M/ME20S antibody
    • ME20S antibody
    • Melanocyte lineage specific antigen GP100 antibody
    • Melanocyte protein mel 17 antibody
    • Melanocyte protein Pmel 17 antibody
    • Melanocyte protein Pmel 17 precursor antibody
    • Melanocytes lineage-specific antigen GP100 antibody
    • Melanoma associated ME20 antigen antibody
    • Melanoma gp100 antibody
    • Melanoma-associated ME20 antigen antibody
    • Melanosomal matrix protein 17 antibody
    • Melanosomal matrix protein17 antibody
    • P1 antibody
    • p100 antibody
    • p26 antibody
    • PMEL 17 antibody
    • PMEL antibody
    • PMEL_HUMAN antibody
    • PMEL17 antibody
    • Premelanosome protein antibody
    • Secreted melanoma-associated ME20 antigen antibody
    • SI antibody
    • SIL antibody
    • SILV antibody
    • Silver (mouse homolog) like antibody
    • Silver homolog antibody
    • Silver locus protein homolog antibody
    • Silver, mouse, homolog of antibody
    see all

Anti-Melanoma gp100 antibody 画像

  • Anti-Melanoma gp100 antibody (ab116582) at 1/100 dilution + CEM cell line lysate at 35 µg

    Predicted band size : 70 kDa

Anti-Melanoma gp100 antibody (ab116582) 使用論文

ab116582 has not yet been referenced specifically in any publications.

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