製品の概要

  • 製品名
    Anti-Melanoma antibody [HMB45]
    Melanoma 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [HMB45] to Melanoma
  • アプリケーション
    適用あり: Flow Cyt, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Tissue, cells or virus corresponding to Human Melanoma. BALB/C mice were injected with extract of pigmented malanoma metastases from lymph nodes.
    Database link: P40967

  • ポジティブ・コントロール
    • Melanoma

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium azide
    Constituents: Tissue culture supernatant, 0.05% BSA
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    HMB45
  • アイソタイプ
    IgG1
  • 軽鎖の種類
    kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/10.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 0.5 - 1 µg/ml.

Incubate for 30 minutes at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

ICC/IF Use a concentration of 0.5 - 1 µg/ml. PubMed: 19841138

ターゲット情報

  • 関連性
    Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre existing benign nevus, which occurs most often in the skin but also may involve other sites.
  • 細胞内局在
    Membrane; Single-pass membrane protein
  • 参照データベース
  • 別名
    • Antigen LB39 AA antibody
    • Antigen SK29 AA antibody
    • MART1 antibody
    • melan A antibody
    • Melan A protein antibody
    • Melanoma antigen recognized by T-cells 1 antibody
    • Monophenol monooxygenase antibody
    • Tumor rejection antigen AB antibody
    • tyrosinase antibody
    see all

画像

  • ab787 - immunohistochemistry

    Formalin fixed paraffin embedded human melanoma stained with ab787, using ABC and AEC chromogen.

  • ab787 staining Melanoma in the human Melanoma cell line WM3248 xenopraph by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue samples were fixed with formaldehyde; permeabilized with 0.1% Triton X-100 and blocked with 100% Dakocytomation X0909 for 1 hour at room temperature; antigen retrival was by heat mediation in Citric buffer (pH6). The sample was incubated with primary antibody (1/25) at 4°C for 18 hours. An HRP-conjugated Goat polyclonal to mouse IgG (1/100) was used as secondary antibody.

    See Abreview

  • Overlay histogram showing Malme-3 cells stained with ab787 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab787, 1/10 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Malme-3 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

参考文献

This product has been referenced in:
  • Liu W  et al. Hepatic epithelioid angiomyolipoma is a rare and potentially severe but treatable tumor: A report of three cases and review of the literature. Oncol Lett 11:3669-3675 (2016). IHC ; Human . Read more (PubMed: 27313680) »
  • Zhu L  et al. Surgical monotherapy may be a suitable therapeutic strategy for advanced collecting (Bellini) duct carcinoma: A case report and literature review. Exp Ther Med 12:1181-1184 (2016). IHC-P ; Human . Read more (PubMed: 27446340) »

See all 11 Publications for this product

レビューと Q&A



We don't have strict guidelines for length of storage for antibodies. In general, for antibody products stored at 4 degrees C, we would expect them to be stable for at least one year from date of purchase.

Merci de nous avoir contactés; ma collègue m'a fait part de votre appel.

Je suis heureux de savoir que l'anticorps a finalement fonctionné en cytométrie en flux. J'ai compris que vous deviez utiliser beaucoup d'anti...

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Thank you for contacting us.

Unfortunately, the blocking peptide for ab787 antibody is not available for purchase. We have ˜25 anti melanoma antibodies available but we do not have antigen available for any one of these however if you could se...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Melanoma cell line WM3248 xenopraph)
Specification
Melanoma cell line WM3248 xenopraph
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric buffer pH6.0
Permeabilization
Yes - 0.1% triton x-100
Blocking step
Dakocytomation x0909 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Username

Hongwei Shao

Verified customer

投稿 Feb 25 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - other (transfected HeLa)
Loading amount
20 µg
Specification
transfected HeLa
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Guillaume Van Niel

Verified customer

投稿 Jul 18 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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