Anti-Melanoma 抗体 [Cocktail - HMB45+A103+T311], prediluted (ab82762)

製品の概要

  • 製品名Anti-Melanoma antibody [Cocktail - HMB45+A103+T311], prediluted
    Melanoma 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [Cocktail - HMB45+A103+T311] to Melanoma, prediluted
  • 特異性This antibody has been shown to label the majority of melanomas.
  • アプリケーション適用あり: IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Other Immunogen Type corresponding to Melanoma. Pigmented melanoma metastases from lymph nodes (HMB45). Recombinant human MART-1 protein (A103). Recombinant tyrosinase protein T311.

  • ポジティブ・コントロール
    • Metastatic melanoma in lymph node.
  • 特記事項

    Isotypes: IgG1/kappa, IgG1 and IgG2a.
    The HMB45 clone reacts with a neuraminidase sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45 reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells and is thought to be oncofetal in nature. Clone A103 recognizes a protein of 20 kDa, identified as MART1 (melanoma antigen recognized by T cells 1) or MelanA. MelanA is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Studies have also shown that MART1 is more sensitive than HMB45 when labeling metastatic melanomas. Tyrosinase is a key enzyme involved in the initial stages of melanin biosynthesis. Studies have shown tyrosinase to be a more sensitive marker when compared to HMB45 and MART1. It has been shown to label a higher percentage of desmoplastic melanomas than HMB45. The combination of HMB45, MART1 and tyrosinase make this triple antibody cocktail a first order pan melanoma screener.

製品の特性

  • 製品の状態Prediluted
  • 保存方法Shipped at 4°C. Store at +4°C.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, Stabilizing agent, pH 7.5
  • Concentration information loading...
  • 一次抗体 備考Isotypes: IgG1/kappa, IgG1 and IgG2a.
    The HMB45 clone reacts with a neuraminidase sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45 reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells and is thought to be oncofetal in nature. Clone A103 recognizes a protein of 20 kDa, identified as MART1 (melanoma antigen recognized by T cells 1) or MelanA. MelanA is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Studies have also shown that MART1 is more sensitive than HMB45 when labeling metastatic melanomas. Tyrosinase is a key enzyme involved in the initial stages of melanin biosynthesis. Studies have shown tyrosinase to be a more sensitive marker when compared to HMB45 and MART1. It has been shown to label a higher percentage of desmoplastic melanomas than HMB45. The combination of HMB45, MART1 and tyrosinase make this triple antibody cocktail a first order pan melanoma screener.
  • ポリ/モノモノクローナル
  • クローン名Cocktail - HMB45+A103+T311
  • アイソタイプIgG1
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/1.

ターゲット情報

  • 関連性Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre existing benign nevus, which occurs most often in the skin but also may involve other sites.
  • 細胞内局在Membrane; Single-pass membrane protein
  • 参照データベース
  • 別名
    • Antigen LB39 AA antibody
    • Antigen SK29 AA antibody
    • MART1 antibody
    • melan A antibody
    • Melan A protein antibody
    • Melanoma antigen recognized by T-cells 1 antibody
    • Monophenol monooxygenase antibody
    • Tumor rejection antigen AB antibody
    • tyrosinase antibody
    see all

Anti-Melanoma antibody [Cocktail - HMB45+A103+T311], prediluted (ab82762) 使用論文

This product has been referenced in:
  • Kapur RP  et al. Anti-melanoma monoclonal antibody HMB45 identifies an oncofetal glycoconjugate associated with immature melanosomes. J Histochem Cytochem 40:207-12 (1992). IHC-P, IHC-Fr . Read more (PubMed: 1552165) »
  • Gown AM  et al. Monoclonal antibodies specific for melanocytic tumors distinguish subpopulations of melanocytes. Am J Pathol 123:195-203 (1986). IHC-P, IHC-Fr . Read more (PubMed: 3518473) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"