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RabMAb

Anti-MEKK3 抗体 [ep600y] (ab40756)

製品の概要

  • 製品名
    Anti-MEKK3 antibody [ep600y]
    MEKK3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [ep600y] to MEKK3
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WB, Flow Cyt, IPmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide within Human MEKK3 aa 600-700 (C terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • HeLa cells, ovary carcinoma
  • 特記事項

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab40756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100 - 1/250.
IHC-P Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 71 kDa).
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/20.

ターゲット情報

  • 機能
    Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.
  • 配列類似性
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 OPR domain.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • M3K3_HUMAN antibody
    • MAP/ERK kinase kinase 3 antibody
    • MAP3K3 antibody
    • MAPK/ERK kinase kinase 3 antibody
    • MAPKKK3 antibody
    • MEK kinase 3 antibody
    • MEKK 3 antibody
    • MEKK3 antibody
    • Mitogen activated protein kinase kinase kinase 3 antibody
    • Mitogen-activated protein kinase kinase kinase 3 antibody
    see all

画像

  • All lanes : Anti-MEKK3 antibody [ep600y] (ab40756) at 1/1000 dilution

    Lane 1 : Recombinant human MEKK2 protein (ab135009)
    Lane 2 : Recombinant human MEKK3 protein (ab85297)

    Lysates/proteins at 0.015 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 71 kDa
    Observed band size: 97 kDa (why is the actual band size different from the predicted?)


    Exposure time: 3 minutes


    Blocking and diluting buffer: 5% NFDM/TBST

  • Immunofluorescent staining of HeLa cells using ab40756 diluted 1:100
  • Anti-MEKK3 antibody [ep600y] (ab40756) at 1/1000 dilution + HeLa cell lysate at 10 µg

    Predicted band size: 71 kDa
    Observed band size: 70 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using ab40756 diluted 1:100

参考文献

This product has been referenced in:

See all 5 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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