製品の概要

  • 製品名Anti-MEK6 antibody [EP557Y]
    MEK6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP557Y] to MEK6
  • 特異性ab33866 recognises MKK6.
  • アプリケーション適用あり: WB, IPmore details
    適用なし: Flow Cyt,ICC or IHC
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in the N terminal of human MKK6.

  • ポジティブ・コントロール
    • Hela cell lysate
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab33866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
IP 1/50.
  • 追加情報Is unsuitable for Flow Cyt,ICC or IHC.
  • ターゲット情報

    • 機能Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively.
    • 組織特異性Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas.
    • 配列類似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Weakly autophosphorylated.
      Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Information by UniProt
    • 参照データベース
    • 製品の状態It localizes to nucleus, cytoplasm and cytoskeleton. Note: Binds to microtubules.
    • 別名
      • Dual specificity mitogen activated protein kinase kinase 6 antibody
      • Dual specificity mitogen-activated protein kinase kinase 6 antibody
      • MAP kinase kinase 6 antibody
      • MAP2K6 antibody
      • MAPK/ERK kinase 6 antibody
      • MAPKK 6 antibody
      • MAPKK6 antibody
      • MEK 6 antibody
      • MEK6 antibody
      • Mitogen Activated Protein Kinase Kinase 6 antibody
      • MKK 6 antibody
      • MKK6 antibody
      • MP2K6_HUMAN antibody
      • PRKMK6 antibody
      • Protein kinase mitogen activated kinase 6 antibody
      • protein kinase, mitogen-activated, kinase 6 (MAP kinase kinase 6) antibody
      • SAPK kinase 3 antibody
      • SAPKK-3 antibody
      • SAPKK3 antibody
      • SKK3 antibody
      • Stress-activated protein kinase kinase 3 antibody
      see all

    Anti-MEK6 antibody [EP557Y] 画像



    • Predicted band size : 37 kDa

      Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)

      Lanes 2, 6 and 10: MEK6 knockout HAP1 cell lysate (20 µg)

      Lanes 3, 7 and 11: HeLa cell lysate (20 µg)

      Lanes 4, 8 and 12: A431 cell lysate (20 µg)

      Lanes 1, 2, 3 and 4: Green signal from target – ab33866 observed at 37 kDa

      Lanes 5, 6, 7 and 8: Red signal from loading control – ab18058 observed at 130 kDa

      Lanes 9, 10, 11 and 12: Merged (red and green) signal

      ab33866 was shown to specifically react with MEK6 when MEK6 knockout samples were used. Wild-type and MEK6 knockout samples were subjected to SDS-PAGE. ab33866 and ab18058 (loading control to Vinculin) were diluted 1/10 000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Anti-MEK6 antibody [EP557Y] (ab33866) at 1/10000 dilution + Hela cell lysate

      Predicted band size : 37 kDa
      Observed band size : 36 kDa (why is the actual band size different from the predicted?)

    Anti-MEK6 antibody [EP557Y] (ab33866) 使用論文

    ab33866 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab33866.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"