Anti-MEK5 抗体 [EP648Y] (ab45146)

製品の概要

  • 製品名Anti-MEK5 antibody [EP648Y]
    MEK5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP648Y] to MEK5
  • アプリケーション適用あり: WB, ICC/IFmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to residues within human MEK5.

  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45146 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC/IF
  • 追加情報ICC/IF: 1/100 - 1/250.

    WB: 1/10000. Predicted molecular weight: 49 kDa.

    Is unsuitable for IHC, FACS or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Acts as a scaffold for the formation of a ternary MAP3K2/MAP3K3-MAP3K5-MAPK7 signaling complex. Activation of this pathway appear to play a critical role in protecting cells from stress-induced apopotosis, neuronal survival and cardiac development and angiogenesis.
    • 組織特異性Expressed in many adult tissues. Abundant in heart and skeletal muscle.
    • 配列類似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 OPR domain.
      Contains 1 protein kinase domain.
    • ドメインBinds MAP3K2/MAP3K3 and MAPK7 via non-overlapping residues of the OPR domain. This domain also mediates interactions with SQSTM1 and PARD6A.
    • 翻訳後修飾Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
      Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Dual specificity mitogen activated protein kinase kinase 5 antibody
      • Dual specificity mitogen-activated protein kinase kinase 5 antibody
      • EC 2.7.12.2 antibody
      • HsT17454 antibody
      • MAP kinase kinase 5 antibody
      • MAP kinase kinase MEK5b antibody
      • MAP2K5 antibody
      • MAPK/ERK kinase 5 antibody
      • MAPKK 5 antibody
      • MAPKK5 antibody
      • MEK 5 antibody
      • mitogen-activated protein kinase kinase 5 antibody
      • MKK5 antibody
      • MP2K5_HUMAN antibody
      • PRKMK5 antibody
      • Protein kinase, mitogen-activated, kinase 5 antibody
      • SAPKK5 antibody
      • SKK5 antibody
      see all

    Anti-MEK5 antibody [EP648Y] 画像

    • Anti-MEK5 antibody [EP648Y] (ab45146) at 1/10000 dilution + HeLa cell lysate

      Predicted band size : 49 kDa
      Observed band size : 49 kDa
    • Immunofluorescent staining of HeLa using ab45146.
    •  ab45146 at 1/100 staining HeLa cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with goat serum prior to incubation with the antibody for 45 minutes. An Alexa Fluor® 488 conjugated goat polyclonal antibody was used as the secondary. This antibody detects MEK5 in HeLa cells as a cytoplasmic protein.

      See Abreview

    Anti-MEK5 antibody [EP648Y] (ab45146) 使用論文

    ab45146 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. I am sorry you are experiencing difficulty with this antibody. Typically when there are black dots on the blot, the antibody is binding with the blocking agent and it is necessary to filter the blocking agent. Was the same ...

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    Thank you for contacting us.

    I understand your concerns about the quality of the antibody. I can confirm, that we would expect the antibody to work well. However, if you experience any problems with this product please do not hesitate and come...

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    Thank you for your enquiry.

    I can confirm that ab45146 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on ...

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    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa, passage 78)
    Loading amount 20 µg
    Specification HeLa, passage 78
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Username

    Abcam user community

    Verified customer

    投稿 Apr 13 2007

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa, passage 78)
    Specification HeLa, passage 78
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 40 minute(s) · Concentration: 3%
    Username

    Abcam user community

    Verified customer

    投稿 Apr 05 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"