Rabbit monoclonal [EP1075Y] to MEK4 (phospho S80)
A synthetic peptide corresponding to residues near the N-terminal of human MKK4
- 3T3 cell lysates (untreated or treated with PDGF) and human muscle tissue.
A trial size is available to purchase for this antibody.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Tissue culture supernatant
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
||1/5000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
Is unsuitable for Flow Cyt or ICC.
Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
Information by UniProt
- c Jun N terminal kinase kinase 1 antibody
- C-JUN N-terminal kinase kinase 1 antibody
- Dual specificity mitogen activated protein kinase kinase 4 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK4 (phospho S80) antibody [EP1075Y] (ab52958)
ab52958 at 1/50 dilution staining MKK4 in human muscles by Immunohistochemistry, Paraffin embedded tissue.
Western blot - MKK4 (phospho S80) antibody [EP1075Y] (ab52958)
All lanes : Anti-MEK4 (phospho S80) antibody [EP1075Y] (ab52958) at 1/5000 dilution
Lane 1 : 3T3 cell lysate untreated
Lane 2 : 3T3 cell lysate treated with PDGF
Lysates/proteins at 10 µg per lane.
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 44 kDa
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"