製品の概要

  • 製品名
    Anti-MEK4 antibody [EP615Y]
    MEK4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP615Y] to MEK4
  • アプリケーション
    適用あり: WBmore details
    適用なし: ICC
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human MEK4 (C terminal).

  • ポジティブ・コントロール
    • HeLa, HEK293, F9, C6, NIH/3T3 cell lysates.
  • 特記事項

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 精製度
    Protein A purified
  • 特記事項(精製)
    ab33912 is a purified IgG.
  • ポリ/モノ
    モノクローナル
  • クローン名
    EP615Y
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab33912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 44 kDa.
  • 追加情報
    Is unsuitable for ICC.
  • ターゲット情報

    • 機能
      Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
    • 組織特異性
      Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
    • 配列類似性
      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻訳後修飾
      Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
    • Information by UniProt
    • 参照データベース
    • 別名
      • c Jun N terminal kinase kinase 1 antibody
      • C-JUN N-terminal kinase kinase 1 antibody
      • Dual specificity mitogen activated protein kinase kinase 4 antibody
      • Dual specificity mitogen-activated protein kinase kinase 4 antibody
      • JNK Activated Kinase 1 antibody
      • JNK activating kinase 1 antibody
      • JNK-activating kinase 1 antibody
      • JNKK antibody
      • JNKK1 antibody
      • MAP kinase kinase 4 antibody
      • Map2k4 antibody
      • MAPK ERK kinase 4 antibody
      • MAPK/ERK kinase 4 antibody
      • MAPKK 4 antibody
      • MAPKK4 antibody
      • MEK 4 antibody
      • MEK4 antibody
      • Mitogen activated protein kinase kinase 4 antibody
      • MKK 4 antibody
      • MKK4 antibody
      • MP2K4_HUMAN antibody
      • PRKMK4 antibody
      • SAPK ERK kinase 1 antibody
      • SAPK/ERK kinase 1 antibody
      • SAPKK 1 antibody
      • SAPKK1 antibody
      • SEK1 antibody
      • SERK1 antibody
      • SKK1 antibody
      • Stress activated protein kinase kinase 1 antibody
      see all

    画像



    • Predicted band size : 44 kDa

      Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
      Lanes 2, 6 and 10: MEK4 knockout HAP1 cell lysate (20 µg)
      Lanes 3, 7 and 11: HeLa cell lysate (20 µg)
      Lanes 4, 8 and 12: A431 cell lysate (20 µg)
      Lanes 1, 2, 3 and 4: Green signal from target - ab33912 observed at 44 kDa
      Lanes 5, 6, 7 and 8: Red signal from loading control - ab8245 observed at 37 kDa
      Lanes 9, 10, 11 and 12: Merged (red and green) signal

      ab33912 was shown to specifically react with MEK4 when MEK4 knockout samples were used. Wild-type and MEK4 knockout samples were subjected to SDS-PAGE. ab33912 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-MEK4 antibody [EP615Y] (ab33912) at 1/5000 dilution (purified)

      Lane 1 : F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysate at 15 µg
      Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg
      Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 44 kDa
      Observed band size : 44 kDa

      Blocking and diluting buffer: 5% NFDM/TBST

    • All lanes : Anti-MEK4 antibody [EP615Y] (ab33912) at 1/1000 dilution (purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
      Lane 2 : 293 (Human embryonic kidney epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 44 kDa
      Observed band size : 44 kDa

      Blocking and diluting buffer: 5% NFDM /TBST

    • Anti-MEK4 antibody [EP615Y] (ab33912) at 1/1000 dilution (unpurified) + HeLa cell lysate

      Predicted band size : 44 kDa
      Observed band size : 44 kDa

    参考文献

    This product has been referenced in:
    • Lukey MJ  et al. The oncogenic transcription factor c-Jun regulates glutaminase expression and sensitizes cells to glutaminase-targeted therapy. Nat Commun 7:11321 (2016). Read more (PubMed: 27089238) »
    • Fey D  et al. Signaling pathway models as biomarkers: Patient-specific simulations of JNK activity predict the survival of neuroblastoma patients. Sci Signal 8:ra130 (2015). Read more (PubMed: 26696630) »

    See all 3 Publications for this product

    レビューと Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (LnCap, PC3, HeLa cells)
    Loading amount
    10 µg
    Specification
    LnCap, PC3, HeLa cells
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    Sigma-AldrichWestern Blocker Solution (W0138) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
    Username

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    Verified customer

    投稿 Nov 01 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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