製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MEK4
  • 特異性
    ab39648 detects endogenous levels of total SEK1/MKK4 protein.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (non-phosphorylated) derived from Human SEK1/MKK4 around the phosphorylation site of Serine 80.

  • ポジティブ・コントロール
    • NIH/3T3 cell extracts.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Affinity chromatography using epitope specific immunogen.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab39648 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
ELISA 1/20000.

ターゲット情報

  • 機能
    Dual specificity kinase that activates the JUN kinases MAPK8 (JNK1) and MAPK9 (JNK2) as well as MAPK14 (p38) but not MAPK1 (ERK2) or MAPK3 (ERK1).
  • 組織特異性
    Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
  • 配列類似性
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Activated by phosphorylation on Ser/Thr by MAP kinase kinase kinases.
  • Information by UniProt
  • 参照データベース
  • 別名
    • c Jun N terminal kinase kinase 1 antibody
    • C-JUN N-terminal kinase kinase 1 antibody
    • Dual specificity mitogen activated protein kinase kinase 4 antibody
    • Dual specificity mitogen-activated protein kinase kinase 4 antibody
    • JNK Activated Kinase 1 antibody
    • JNK activating kinase 1 antibody
    • JNK-activating kinase 1 antibody
    • JNKK antibody
    • JNKK1 antibody
    • MAP kinase kinase 4 antibody
    • Map2k4 antibody
    • MAPK ERK kinase 4 antibody
    • MAPK/ERK kinase 4 antibody
    • MAPKK 4 antibody
    • MAPKK4 antibody
    • MEK 4 antibody
    • MEK4 antibody
    • Mitogen activated protein kinase kinase 4 antibody
    • MKK 4 antibody
    • MKK4 antibody
    • MP2K4_HUMAN antibody
    • PRKMK4 antibody
    • SAPK ERK kinase 1 antibody
    • SAPK/ERK kinase 1 antibody
    • SAPKK 1 antibody
    • SAPKK1 antibody
    • SEK1 antibody
    • SERK1 antibody
    • SKK1 antibody
    • Stress activated protein kinase kinase 1 antibody
    see all

画像

  • Anti-MEK4 antibody (ab39648) at 1/3000 dilution + Mouse small intestinal epithelial whole cell lysates at 15 µg

    Secondary
    Goat anti-rabbit polyclonal antibody at 1/7000 dilution
    Developed using the ECL technique

    Predicted band size : 44 kDa
    Observed band size : 43 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 38 kDa (possible non-specific binding).

    Exposure time : 40 seconds

    This image is courtesy of an anonymous abreview.

    Blocking: 5% milk for 1 hour at 25°C. Dilutent: TBS-TWEEN, incubated for 12 hours at 4°C.

    See Abreview

  • All lanes : Anti-MEK4 antibody (ab39648) at 1/500 dilution

    Lane 1 : NIH/3T3 cell extracts 5-30µg/lane
    Lane 2 : NIH/3T3 cell extracts 5-30µg/lane with blocking peptide at 1 µg/ml


    Predicted band size : 44 kDa
    Observed band size : 44 kDa

プロトコール

参考文献

ab39648 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Small Intestinal Epithelial)
Loading amount
15 µg
Specification
Small Intestinal Epithelial
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Mar 06 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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