製品の概要

  • 製品名Anti-MEK3 (phospho T222) antibody
    MEK3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MEK3 (phospho T222)
  • 特異性ab79586 detects endogenous levels of MEK3 only when phosphorylated at threonine 222.
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human MEK3 around the phosphorylation site of threonine 222 (A-K-TP-M-D)

  • ポジティブ・コントロール
    • Human brain tissue; Jurkat cell extracts treated with serum (20 %, 15 minuutes).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 39 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
  • 組織特異性Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
  • 関連疾患Note=Defects in MAP2K3 may be involved in colon cancer.
  • 配列類似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Autophosphorylated.
    Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
    Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AW212142 antibody
    • dual specificity mitogen activated protein kinase kinase 3 antibody
    • Dual specificity mitogen-activated protein kinase kinase 3 antibody
    • MAP kinase kinase 3 antibody
    • map2k3 antibody
    • MAPK ERK kinase 3 antibody
    • MAPK/ERK kinase 3 antibody
    • MAPKK 3 antibody
    • MAPKK3 antibody
    • MEK 3 antibody
    • MEK3 antibody
    • Mitogen activated protein kinase kinase 3 antibody
    • MKK 3 antibody
    • MKK3 antibody
    • mMKK3b antibody
    • MP2K3_HUMAN antibody
    • PRKMK 3 antibody
    • PRKMK3 antibody
    • protein kinase, mitogen-activated, kinase 3 antibody
    • SAPK kinase 2 antibody
    • SAPKK 2 antibody
    • SAPKK2 antibody
    • Stress activated protein kinase kinase 2 antibody
    see all

Anti-MEK3 (phospho T222) antibody 画像

  • ICC/IF image of ab79586 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79586, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-MEK3 (phospho T222) antibody (ab79586) at 1/500 dilution

    Lane 1 : Jurkat cell extracts treated with serum (20 %, 15 minutes)
    Lane 2 : Jurkat cell extracts treated with serum (20 %, 15 minutes) with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 39 kDa
    Observed band size : 39 kDa
    Additional bands at : 32 kDa. We are unsure as to the identity of these extra bands.
  • ab79586, at a 1/50 dilution, staining MEK3 in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing peptide.

Anti-MEK3 (phospho T222) antibody (ab79586) 使用論文

ab79586 has not yet been referenced specifically in any publications.

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