製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MEK3
  • アプリケーション
    適用あり: IHC-P, ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide:

    MESPASSQPASMPQSK

    conjugated to KLH, corresponding to N terminal amino acids 1-16 of Human MKK3

  • ポジティブ・コントロール
    • Jurkat cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab5428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 35 kDa (predicted molecular weight: 38 kDa).

ターゲット情報

  • 機能
    Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38.
  • 組織特異性
    Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.
  • 関連疾患
    Note=Defects in MAP2K3 may be involved in colon cancer.
  • 配列類似性
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    Autophosphorylated.
    Phosphorylation on Ser-218 and Thr-222 by MAP kinase kinase kinases regulates positively the kinase activity.
    Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AW212142 antibody
    • dual specificity mitogen activated protein kinase kinase 3 antibody
    • Dual specificity mitogen-activated protein kinase kinase 3 antibody
    • MAP kinase kinase 3 antibody
    • map2k3 antibody
    • MAPK ERK kinase 3 antibody
    • MAPK/ERK kinase 3 antibody
    • MAPKK 3 antibody
    • MAPKK3 antibody
    • MEK 3 antibody
    • MEK3 antibody
    • Mitogen activated protein kinase kinase 3 antibody
    • MKK 3 antibody
    • MKK3 antibody
    • mMKK3b antibody
    • MP2K3_HUMAN antibody
    • PRKMK 3 antibody
    • PRKMK3 antibody
    • protein kinase, mitogen-activated, kinase 3 antibody
    • SAPK kinase 2 antibody
    • SAPKK 2 antibody
    • SAPKK2 antibody
    • Stress activated protein kinase kinase 2 antibody
    see all

画像



  • Predicted band size : 38 kDa

    ab5428 at a 1/100 dilution staining approximately 35kDa band of MKK 3 in Jurkat cell lysate by Western blot (ECL). ab5428 at a 1/100 dilution staining approximately 35kDa band of MKK 3 in Jurkat cell lysate by Western blot (ECL).  Secondary antibody - anti-rabbit HRP

  • ab5428 staining MEK3 in human breast carcinoma (BC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

参考文献

ab5428 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - other (brain tissue lysate, cytosolic fraction)
Loading amount
10 µg
Specification
brain tissue lysate, cytosolic fraction
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Username

Dr. Maryna Polyakova

Verified customer

投稿 May 09 2012

Thank you for your reply.
Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these addition...

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Thank you very much for your interest in our antibodies.

We appreciate receiving feedback and data from customers, and we do have a testing discount offer available.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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