Anti-MEK2 抗体 [Y78] (ab32517)

製品の概要

製品名

Anti-MEK2 antibody [Y78]
MEK2 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [Y78] to MEK2
特異性

This antibody does not cross react with other MAP kinase kinase family members
アプリケーション

適用あり: ICC/IF, WB, IHC-P, Flow Cyt, IPmore details
種交差性

交差種: Mouse, Human
免疫原

Synthetic peptide. within Human MEK2 aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P36507
ポジティブ･コントロール
- WB: Jurkat cell lysate. ICC/IF: HeLa and wildtype HAP1 cells. IHC-P: Human prostate carcinoma tissue.
特記事項

This product is a recombinant rabbit monoclonal antibody.

A trial size is available to purchase for this antibody.

Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Alternative versions available:
Anti-MEK2 antibody (Alexa Fluor^® 488) [Y78] (ab200606)

Anti-MEK2 antibody (Alexa Fluor^® 647) [Y78] (ab200519)

Anti-MEK2 antibody (HRP) [Y78] (ab200607)
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents

製品の特性

製品の状態

Liquid
保存方法

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファー

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

Y78
アイソタイプ

IgG
研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab32517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション	Abreviews	特記事項
ICC/IF		Use a concentration of 1 µg/ml.
WB		1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).
IHC-P		Use at an assay dependent concentration.
Flow Cyt		1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP		1/100.

ターゲット情報

機能

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
関連疾患

Defects in MAP2K2 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
配列類似性

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
Contains 1 protein kinase domain.
翻訳後修飾

MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).
Acetylation of Ser-222 and Ser-226 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
Target information above from: UniProt accession P36507 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 5605 Human
- Entrez Gene: 26396 Mouse
- Omim: 601263 Human
- SwissProt: P36507 Human
- SwissProt: Q63932 Mouse
- Unigene: 465627 Human
- Unigene: 275436 Mouse
別名
- Cardiofaciocutaneous syndrome antibody
- CFC syndrome antibody
- CFC4 antibody
- Dual specificity mitogen activated protein kinase kinase 2 antibody
- Dual specificity mitogen-activated protein kinase kinase 2 antibody
- ERK activator kinase 2 antibody
- FLJ26075 antibody
- MAP kinase kinase 2 antibody
- map2k2 antibody
- MAPK / ERK kinase 2 antibody
- MAPK/ERK kinase 2 antibody
- MAPKK 2 antibody
- MAPKK2 antibody
- MEK 2 antibody
- MEK2 antibody
- Microtubule associated protein kinase kinase 2 antibody
- Mitogen activated protein kinase kinase 2 antibody
- Mitogen activated protein kinase kinase 2 p45 antibody
- MKK 2 antibody
- MKK2 antibody
- MP2K2_HUMAN antibody
- OTTHUMP00000165826 antibody
- OTTHUMP00000165827 antibody
- PRKMK 2 antibody
- PRKMK2 antibody
see all

Anti-MEK2 antibody [Y78] 画像

Western blot - Anti-MEK2 antibody [Y78] (ab32517)

Predicted band size : 44 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MEK2 knockout HAP1 cell lysate (20 µg)

Lane 3: Jurkat cell lysate (20 µg)
Lane 4: K562 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32517 observed at 44 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab32517 was shown to specifically react with MEK2 when MEK2 knockout samples were used. Wild-type and MEK2 knockout samples were subjected to SDS-PAGE. ab32517 and ab8245 (loading control to GAPDH) were diluted 1/10 000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Immunocytochemistry/ Immunofluorescence - Anti-MEK2 antibody [Y78] (ab32517)

ab32517 staining MEK2 in wild-type HAP1 cells (top panel) and MEK2 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab32517 at 1μg/ml and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Western blot - Anti-MEK2 antibody [Y78] (ab32517)

All lanes : Anti-MEK2 antibody [Y78] (ab32517) at 1/10000 dilution

Lane 1 : Jurkat Whole Cell Lysate
Lane 2 : Mouse Brain Tissue Lysate
Lane 3 : Mouse Lung Tissue Lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 44 kDa
Observed band size : 44 kDa
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using Licor blocking buffer before being incubated with ab32517 overnight at 4°C. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) at a 1:10000 dilution for 1hr at room temperature and then imaged.
Western blot - MEK2 antibody [Y78] (ab32517)

Anti-MEK2 antibody [Y78] (ab32517) at 1/10000 dilution + Jurkat cell lysate

Predicted band size : 44 kDa
Observed band size : 45 kDa (why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - MEK2 antibody [Y78] (ab32517)

Immunofluorescent staining of HeLa cells using ab32517 at a dilution of 1/250.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEK2 antibody [Y78] (ab32517)

Immunohistochemical analysis of paraffin embedded prostate carcinoma using ab32517 at a dilution of 1/250.
Flow Cytometry - MEK2 antibody [Y78] (ab32517)

Overlay histogram showing HeLa cells stained with ab32517 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32517, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Anti-MEK2 antibody [Y78] (ab32517) 使用論文

This product has been referenced in:

Li L et al. MEK1 promotes YAP and their interaction is critical for tumorigenesis in liver cancer. FEBS Lett 587:3921-7 (2013). WB . Read more (PubMed: 24211253) »
Yamashita S et al. The Leydig cell MEK/ERK pathway is critical for maintaining a functional population of adult Leydig cells and for fertility. Mol Endocrinol 25:1211-22 (2011). WB ; Mouse . Read more (PubMed: 21527500) »