製品の概要

  • 製品名
    Anti-MEK2 antibody [1A1]
    MEK2 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1A1] to MEK2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    非交差種: Mouse, Rat
  • 免疫原

    Recombinant human protein purified from E.coli (His-MEK2)

  • ポジティブ・コントロール
    • WB: HeLa, U87MG, MCF7, and U937 cell lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Detects a band of approximately 47 kDa (predicted molecular weight: 44 kDa).

ターゲット情報

  • 機能
    Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
  • 関連疾患
    Defects in MAP2K2 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
  • 配列類似性
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾
    MAPKK is itself dependent on Ser/Thr phosphorylation for activity catalyzed by MAP kinase kinase kinases (RAF or MEKK1).
    Acetylation of Ser-222 and Ser-226 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cardiofaciocutaneous syndrome antibody
    • CFC syndrome antibody
    • CFC4 antibody
    • Dual specificity mitogen activated protein kinase kinase 2 antibody
    • Dual specificity mitogen-activated protein kinase kinase 2 antibody
    • ERK activator kinase 2 antibody
    • FLJ26075 antibody
    • MAP kinase kinase 2 antibody
    • map2k2 antibody
    • MAPK / ERK kinase 2 antibody
    • MAPK/ERK kinase 2 antibody
    • MAPKK 2 antibody
    • MAPKK2 antibody
    • MEK 2 antibody
    • MEK2 antibody
    • Microtubule associated protein kinase kinase 2 antibody
    • Mitogen activated protein kinase kinase 2 antibody
    • Mitogen activated protein kinase kinase 2 p45 antibody
    • MKK 2 antibody
    • MKK2 antibody
    • MP2K2_HUMAN antibody
    • OTTHUMP00000165826 antibody
    • OTTHUMP00000165827 antibody
    • PRKMK 2 antibody
    • PRKMK2 antibody
    see all

画像



  • Predicted band size : 44 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: MEK2 knockout HAP1 cell lysate (20 µg)
    Lane 3: Jurkat cell lysate (20 µg)
    Lane 4: K562 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab70590 observed at 44 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab70590 was shown to recognize MEK2 when MEK2 knockout samples were used, along with additional cross-reactive bands. Wild-type and MEK2 knockout samples were subjected to SDS-PAGE. ab70590 and ab181602 (loading control to GAPDH) were diluted 1/2000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • All lanes : Anti-MEK2 antibody [1A1] (ab70590) at 1/500 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : U87MG cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : U937 cell lysate


    Predicted band size : 44 kDa

    Incubated with the primary antibody overnight at 4°C. Incubated with the secondary antibody for 1 hour at room temperature.

  • All lanes : Anti-MEK2 antibody [1A1] (ab70590) at 1/1000 dilution

    Lane 1 : MEK1 recombinant protein
    Lane 2 : MEK2 recombinant protein
    Lane 3 : MEK3 recombinant protein
    Lane 4 : MEK4 recombinant protein
    Lane 5 : MEK5 recombinant protein
    Lane 6 : MEK6 recombinant protein
    Lane 7 : MEK7 recombinant protein


    Predicted band size : 44 kDa

    Incubated with the primary antibody overnight at 4°C. Incubated with the secondary antibody for 1 hour at room temperature.

プロトコール

参考文献

ab70590 has not yet been referenced specifically in any publications.

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