Anti-MEK1 (phospho S298) 抗体 (ab5613)

製品の概要

  • 製品名Anti-MEK1 (phospho S298) antibody
    MEK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MEK1 (phospho S298)
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human MEK 1 that contains serine 298.

  • ポジティブ・コントロール
    • NIH3T3 cells +/- PDGF, A431 +/- EGF, PC12 +/- NGF.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated MEK 1. The final product is generated by affinity chromatography using a MEK 1 derived peptide that is phosphorylated at serine 298.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 50 kDa.

ターゲット情報

  • 機能Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
  • 組織特異性Widely expressed, with extremely low levels in brain.
  • 関連疾患Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
  • 配列類似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
    Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dual specificity mitogen activated protein kinase kinase 1 antibody
    • Dual specificity mitogen-activated protein kinase kinase 1 antibody
    • ERK activator kinase 1 antibody
    • MAP kinase kinase 1 antibody
    • MAP kinase/Erk kinase 1 antibody
    • MAP2K1 antibody
    • MAPK/ERK kinase 1 antibody
    • MAPKK 1 antibody
    • MAPKK1 antibody
    • MEK 1 antibody
    • Mek1 antibody
    • MEKK1 antibody
    • Mitogen activated protein kinase kinase 1 antibody
    • MKK 1 antibody
    • MKK1 antibody
    • MP2K1_HUMAN antibody
    • PRKMK1 antibody
    • Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
    • Protein kinase mitogen activated, kinase 1 antibody
    see all

Anti-MEK1 (phospho S298) antibody 画像

  • Peptide Competition: Extracts prepared from NIH3T3 cells treated with PDGF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab5613 antibody for two hours at room temperature, following prior incubation with: the phosphopeptide immunogen (1), the nonphosphopeptide corresponding to the immunogen (2), a generic phosphoserine containing peptide (3), or, no peptide (4). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. Phosphatase Stripping: Extracts prepared from NIH3T3 cells not treated or treated (+PDGF) with PDGF were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was either treated (lane 5) or not treated (lanes 6, 7) with lambda phosphatase, then incubated with 0.50 µg/mL ab5613 antibody for two hours at room temperature. After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG alkaline phosphatase and signals were detected using the Tropix WesternStar method. The data show that only the peptide corresponding to ab5613 blocks the antibody signal, demonstrating the specificity of the antibody. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.

Anti-MEK1 (phospho S298) antibody (ab5613) 使用論文

ab5613 has not yet been referenced specifically in any publications.

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Thank you for your enquiry. I am able to provide you with the following information; for some of the antibodies that you are interested in, immunogen information is considered to be proprietary. Ab7948: The sequence is as follows: IFEETARFQPGYRS ...

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