製品の概要

  • 製品名Anti-MEK1 antibody [E237]
    MEK1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E237] to MEK1
  • アプリケーション適用あり: ICC/IF, WB, IP, IHC-Pmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MEK1.

  • ポジティブ・コントロール
    • WB: EGF treated A431 cells lysate. IHC-P: Skin carcinoma.
  • 特記事項

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32088 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/50 - 1/100.
WB 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 45 kDa).
IP 1/30.
IHC-P 1/50 - 1/100.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
    • 組織特異性Widely expressed, with extremely low levels in brain.
    • 関連疾患Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
    • 配列類似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
      Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Dual specificity mitogen activated protein kinase kinase 1 antibody
      • Dual specificity mitogen-activated protein kinase kinase 1 antibody
      • ERK activator kinase 1 antibody
      • MAP kinase kinase 1 antibody
      • MAP kinase/Erk kinase 1 antibody
      • MAP2K1 antibody
      • MAPK/ERK kinase 1 antibody
      • MAPKK 1 antibody
      • MAPKK1 antibody
      • MEK 1 antibody
      • Mek1 antibody
      • MEKK1 antibody
      • Mitogen activated protein kinase kinase 1 antibody
      • MKK 1 antibody
      • MKK1 antibody
      • MP2K1_HUMAN antibody
      • PRKMK1 antibody
      • Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody
      • Protein kinase mitogen activated, kinase 1 antibody
      see all

    Anti-MEK1 antibody [E237] 画像

    • Antigen: MEK1 (phospho S218 + S222) Peptide; MEK1 non-phospho Peptide

      Antigen concentration: 1000ng/ml

      Primary antibody concentration range: 0~1000ng/ml

      Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 1/2500 dilution

       

    • All lanes : Anti-MEK1 antibody [E237] (ab32088) at 1/1000 dilution

      Lane 1 : Untreated A431 (human epithelial carcinoma) whole cell lysates
      Lane 2 : A431 (human epithelial carcinoma) cells were treated with 100ng/ml Epidermal growth factor (EGF) for 10 minutes whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), HRP conjugated)

      Predicted band size : 45 kDa
      Observed band size : 43 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-MEK1 antibody [E237] (ab32088) at 1/1000 dilution

      Lane 1 : Untreated HeLa (human cervix adenocarcinoma) whole cell lysates
      Lane 2 : HeLa (human cervix adenocarcinoma) cells were treated with 50ng/ml Nocodazole for 20 hours whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), HRP conjugated)

      Predicted band size : 45 kDa
      Observed band size : 43 kDa (why is the actual band size different from the predicted?)


      Exposure time : 5 seconds

      Blocking and dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-MEK1 antibody [E237] (ab32088) at 1/1000 dilution

      Lane 1 : A431 (human epithelial carcinoma) cells were treated with 100ng/ml Epidermal growth factor (EGF) for 10 minutes whole cell lysates plus MEK1 (pS218 + pS222) phospho peptide
      Lane 2 : A431 (human epithelial carcinoma) cells were treated with 100ng/ml Epidermal growth factor (EGF) for 10 minutes whole cell lysates plus MEK1 non-phospho peptide
      Lane 3 : A431 (human epithelial carcinoma) cells were treated with 100ng/ml Epidermal growth factor (EGF) for 10 minutes whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 (Goat Anti-Rabbit IgG, (H+L), HRP conjugated)

      Predicted band size : 45 kDa
      Observed band size : 43 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and dilution buffer: 5% NFDM/TBST

    • All lanes : Anti-MEK1 antibody [E237] (ab32088) at 1/1000 dilution

      Lane 1 : HEK293 (human embryonic kidney) whole cell lysates plus MEK1 (pS218 + pS222) phospho peptide
      Lane 2 : HEK293 (human embryonic kidney) whole cell lysates plus MEK1 non-phospho peptide
      Lane 3 : HEK293 (human embryonic kidney) whole cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), HRP conjugated)

      Predicted band size : 45 kDa
      Observed band size : 43 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

      Blocking and dilution buffer: 5% NFDM/TBST

    • Primary ab Dilution: 1:1000 dilution

      Secondary ab description and code (ab id): Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated 

      Secondary ab dilution: 1:2500 dilution

      Blocking buffer and concentration: 5% NFDM/TBST

      Diluting buffer and concentration: 5% NFDM /TBST

      Lane 1: MEK1 (pS218 + pS222) phospho peptide

      Lane 2: MEK1 non-phospho peptide

      Exposure time: 10 seconds

    • All lanes : Anti-MEK1 antibody [E237] (ab32088) at 1/1000 dilution

      Lane 1 : Serum starved A431 cells lysate.
      Lane 2 : EGF treated serum starved A431 cells. lysate


      Predicted band size : 45 kDa
      Observed band size : 43 kDa (why is the actual band size different from the predicted?)
    • ab32088 staining MEK1 (phospho S218 + S222) in SKNSH cells treated with dopamine hydrochloride (ab120565), by ICC/IF. Increase in MEK1 (phospho S218 + S222) expression correlates with increased concentration of dopamine hydrochloride, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120565 (dopamine hydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32088 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
    • Ab32088, at a 1/50 dilution, staining MEK1 in paraffin embedded skin carcinoma tissue sections by Immunohistochemistry.

    Anti-MEK1 antibody [E237] (ab32088) 使用論文

    This product has been referenced in:
    • Wang Q  et al. Extracellular calumenin suppresses ERK1/2 signaling and cell migration by protecting fibulin-1 from MMP-13-mediated proteolysis. Oncogene N/A:N/A (2014). Read more (PubMed: 24632605) »
    • Cole CL  et al. Ovarian cancer cell heparan sulfate 6-O-sulfotransferases regulate an angiogenic program induced by heparin-binding epidermal growth factor (EGF)-like growth factor/EGF receptor signaling. J Biol Chem 289:10488-501 (2014). WB ; Human . Read more (PubMed: 24563483) »

    See all 7 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HuH7 and HT29 tumor cells)
    Specification HuH7 and HT29 tumor cells
    Blocking step Triton-X-100 PBS supplemented with 10% rat serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 0.5%
    Fixative Paraformaldehyde
    Username

    Paul Dent

    Verified customer

    投稿 Feb 17 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"