Anti-MEIS2 抗体 (ab73164)
Key features and details
- Rabbit polyclonal to MEIS2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-MEIS2 antibody
MEIS2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MEIS2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Horse, Cow, Dog -
免疫原
Synthetic peptide corresponding to Human MEIS2 aa 450 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab73163) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab73164の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).Can be blocked with Human MEIS2 peptide (ab73163).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).Can be blocked with Human MEIS2 peptide (ab73163). |
ターゲット情報
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組織特異性
Expressed in various tissues. In hematopoietic tissues, the lymphoid organs express high levels of MEIS2. Also expressed in some regions of the brain, such as the putamen. -
配列類似性
Belongs to the TALE/MEIS homeobox family.
Contains 1 homeobox DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 539573 Cow
- Entrez Gene: 612759 Dog
- Entrez Gene: 4212 Human
- Entrez Gene: 17536 Mouse
- Entrez Gene: 311311 Rat
- Omim: 601740 Human
- SwissProt: O14770 Human
- SwissProt: P97367 Mouse
see all -
別名
- Homeobox protein Meis2 antibody
- HsT18361 antibody
- Meis (mouse) homolog 2 antibody
see all
画像
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All lanes : Anti-MEIS2 antibody (ab73164) at 1 µg/ml
Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue
Lane 2 : Brain (Mouse) Tissue Lysate - adult normal tissue
Lane 3 : Brain (Rat) Tissue Lysate - adult normal tissue
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab73164 in 2% Milk, overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab73164 は 7 報の論文で使用されています。
- Katreddi RR et al. Notch signaling determines cell-fate specification of the two main types of vomeronasal neurons of rodents. Development 149:N/A (2022). PubMed: 35781337
- Finer G et al. Stromal Transcription Factor 21 Regulates Development of the Renal Stroma via Interaction with Wnt/β-Catenin Signaling. Kidney360 3:1228-1241 (2022). PubMed: 35919523
- Lin JM et al. Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2ε in vomeronasal sensory neurons. Elife 11:N/A (2022). PubMed: 36111787
- Yan G et al. LncRNA ILF3-AS1 promotes cell migration, invasion and EMT process in hepatocellular carcinoma via the miR-628-5p/MEIS2 axis to activate the Notch pathway. Dig Liver Dis N/A:N/A (2021). PubMed: 34053876
- Wang L et al. The transcriptional regulator MEIS2 sets up the ground state for palatal osteogenesis in mice. J Biol Chem 295:5449-5460 (2020). PubMed: 32169905
- Wan Z et al. MEIS2 promotes cell migration and invasion in colorectal cancer. Oncol Rep 42:213-223 (2019). PubMed: 31115559
- Wu YH et al. miR-134 Modulates the Proliferation of Human Cardiomyocyte Progenitor Cells by Targeting Meis2. Int J Mol Sci 16:25199-213 (2015). WB ; Human . PubMed: 26512644