製品の概要

  • 製品名Anti-MEF2D antibody
    MEF2D 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to MEF2D
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length human MEF2D protein with tag

  • ポジティブ・コントロール
    • K-562 cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab60717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

ターゲット情報

  • 機能Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis.
  • 配列類似性Belongs to the MEF2 family.
    Contains 1 MADS-box domain.
    Contains 1 Mef2-type DNA-binding domain.
  • 発生段階Present in myotubes and also in undifferentiated myoblasts.
  • ドメインThe beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity.
  • 翻訳後修飾Phosphorylated on Ser-444 by CDK5 is required for Lys-439 sumoylation and inhibits transcriptional activity. In neurons, enhanced CDK5 activity induced by neurotoxins promotes caspase 3-mediated cleavage leading to neuron apoptosis. Phsophorylation on Ser-180 can be enhanced by EGF.
    Acetylated on Lys-439 by CREBBP. Deacetylated by SIRT1.
    Sumoylated on Lys-439 by SUMO2 but not SUMO1; which inhibits transcriptional activity and myogenic activity. Desumoylated by SENP3.
    Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
  • 細胞内局在Nucleus. Translocated by HDAC4 to nuclear dots.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp686I1536 antibody
    • MADS box transcription factor 2 polypeptide D antibody
    • Mef2d antibody
    • MEF2D_HUMAN antibody
    • Myocyte enhancer factor 2D antibody
    • Myocyte specific enhancer factor 2, polypeptide D antibody
    • Myocyte specific enhancer factor 2D antibody
    • Myocyte-specific enhancer factor 2D antibody
    see all

Anti-MEF2D antibody 画像

  • Anti-MEF2D antibody (ab60717) at 1 µg/ml + K-562 cell lysate at 25 µg

    Predicted band size : 56 kDa
    Observed band size : 56 kDa

Anti-MEF2D antibody (ab60717) 使用論文

This product has been referenced in:
  • Ye J  et al. Translation of Myocyte Enhancer Factor-2 is induced by hypertrophic stimuli in cardiomyocytes through a Calcineurin-dependent pathway. J Mol Cell Cardiol 53:578-87 (2012). Read more (PubMed: 22850285) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"