製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MEF2D
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-521 of Human MEF2D (NP_005911.1).

  • ポジティブ・コントロール
    • Human kidney tissue lysate; MEF2D transfected 293T cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab104515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 56 kDa.

ターゲット情報

  • 機能
    Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific, growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. Plays a critical role in the regulation of neuronal apoptosis.
  • 配列類似性
    Belongs to the MEF2 family.
    Contains 1 MADS-box domain.
    Contains 1 Mef2-type DNA-binding domain.
  • 発生段階
    Present in myotubes and also in undifferentiated myoblasts.
  • ドメイン
    The beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity.
  • 翻訳後修飾
    Phosphorylated on Ser-444 by CDK5 is required for Lys-439 sumoylation and inhibits transcriptional activity. In neurons, enhanced CDK5 activity induced by neurotoxins promotes caspase 3-mediated cleavage leading to neuron apoptosis. Phsophorylation on Ser-180 can be enhanced by EGF.
    Acetylated on Lys-439 by CREBBP. Deacetylated by SIRT1.
    Sumoylated on Lys-439 by SUMO2 but not SUMO1; which inhibits transcriptional activity and myogenic activity. Desumoylated by SENP3.
    Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
  • 細胞内局在
    Nucleus. Translocated by HDAC4 to nuclear dots.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp686I1536 antibody
    • MADS box transcription factor 2 polypeptide D antibody
    • Mef2d antibody
    • MEF2D_HUMAN antibody
    • Myocyte enhancer factor 2D antibody
    • Myocyte specific enhancer factor 2, polypeptide D antibody
    • Myocyte specific enhancer factor 2D antibody
    • Myocyte-specific enhancer factor 2D antibody
    see all

画像

  • Anti-MEF2D antibody (ab104515) at 1 µg/ml + Human kidney tissue lysate at 50 µg

    Predicted band size : 56 kDa
  • All lanes : Anti-MEF2D antibody (ab104515) at 1 µg/ml

    Lane 1 : MEF2D transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 56 kDa

プロトコール

参考文献

ab104515 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Squirrel Tissue lysate - whole (Brown Adipose Tissue)
Specification
Brown Adipose Tissue
Blocking step
BSA as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Mr. James Bjork

Verified customer

投稿 Sep 05 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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