Anti-MEF2C (phospho S396) 抗体 (ab78888)
Key features and details
- Rabbit polyclonal to MEF2C (phospho S396)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MEF2C (phospho S396) antibody
MEF2C 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MEF2C (phospho S396) -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human MEF2C (phospho S396).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from rabbit antiserum by affinity chromatography, using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab78888の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (4) |
1/500 - 1/1000. Predicted molecular weight: 51 kDa.
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IHC-P |
1/50 - 1/100.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 51 kDa. |
IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2. -
組織特異性
Expressed in brain and skeletal muscle. -
関連疾患
Defects in MEF2C are the cause of mental retardation-stereotypic movements-epilepsy and/or cerebral malformations (MRSME) [MIM:613443]. It is a disorder characterized by severe mental retardation, absent speech, hypotonia, poor eye contact and stereotypic movements. Dysmorphic features include high broad forehead with variable small chin, short nose with anteverted nares, large open mouth, upslanted palpebral fissures and prominent eyebrows. Some patients have seizures. -
配列類似性
Belongs to the MEF2 family.
Contains 1 MADS-box domain.
Contains 1 Mef2-type DNA-binding domain. -
発生段階
Expression is highest during the early stages of postnatal development, at later stages levels greatly decrease. -
ドメイン
The beta domain, missing in a number of isoforms, is required for enhancement of transcriptional activity. -
翻訳後修飾
Phosphorylation on Ser-59 enhances DNA binding activity (By similarity). Phosphorylation on Ser-396 is required for Lys-391 sumoylation and inhibits transcriptional activity.
Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation.
Sumoylated on Lys-391 by SUMO2 but not by SUMO1 represses transcriptional activity.
Proteolytically cleaved in cerebellar granule neurons, probably by caspase 7, following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 4208 Human
- Entrez Gene: 17260 Mouse
- Omim: 600662 Human
- SwissProt: Q06413 Human
- SwissProt: Q8CFN5 Mouse
- Unigene: 649965 Human
- Unigene: 24001 Mouse
- Unigene: 451574 Mouse
see all -
別名
- C5DELq14.3 antibody
- DEL5q14.3 antibody
- MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C) antibody
see all
画像
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Immunohistochemistry analysis of paraffin-embedded Human brain tissue using ab78888 at 1/50 dilution in the absence or presence of the immunzing phosphopeptide.
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All lanes : Anti-MEF2C (phospho S396) antibody (ab78888) at 1/500 dilution
Lane 1 : Extracts from 24 hours starved 3T3 cells
Lane 2 : Extracts from 24 hours starved 3T3 cells with immunizing phosphopeptide at 5 µg
Lysates/proteins at 20 µg per lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab78888 は 5 報の論文で使用されています。
- Wu C et al. Mapk7 deletion in chondrocytes causes vertebral defects by reducing MEF2C/PTEN/AKT signaling. Genes Dis 11:964-977 (2024). WB ; Mouse . PubMed: 37692479
- Tidyman WE et al. MEK-inhibitor-mediated rescue of skeletal myopathy caused by activating Hras mutation in a Costello syndrome mouse model. Dis Model Mech 15:N/A (2022). PubMed: 34553752
- Fritz KR et al. Cdc42 activation by endothelin regulates neural crest cell migration in the cardiac outflow tract. Dev Dyn 248:795-812 (2019). PubMed: 31219639
- Chen YT et al. B Cell Development sans B Cell Receptor Responsiveness Due to Unfolded Protein Response-Triggered Mef2c Protein Degradation. J Immunol 201:2885-2898 (2018). PubMed: 30305329
- Chen B et al. Inhibition of miR-29c promotes proliferation, and inhibits apoptosis and differentiation in P19 embryonic carcinoma cells. Mol Med Rep 13:2527-35 (2016). WB ; Mouse . PubMed: 26848028