製品の概要

  • 製品名Anti-MEF2A (phospho S408) antibody
    MEF2A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MEF2A (phospho S408)
  • 特異性This antibody detects endogenous levels of MEF2A only when phosphorylated at serine 408.
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human MEF2A around the phosphorylation site of serine 408 (P-I-SP-P-P).

  • ポジティブ・コントロール
    • Human breast carcinoma tissue, HeLa cell extracts treated with PMA at 125ng/ml for 30 minutes.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab51151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Transcriptional activator which binds specifically to the MEF2 element, 5'-YTA[AT](4)TAR-3', found in numerous muscle-specific genes. Also involved in the activation of numerous growth factor- and stress-induced genes. Mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. Plays diverse roles in the control of cell growth, survival and apoptosis via p38 MAPK signaling in muscle-specific and/or growth factor-related transcription. In cerebellar granule neurons, phosphorylated and sumoylated MEF2A represses transcription of NUR77 promoting synaptic differentiation.
  • 組織特異性Isoform MEF2 and isoform MEFA are expressed only in skeletal and cardiac muscle and in the brain. Isoform RSRFC4 and isoform RSRFC9 are expressed in all tissues examined.
  • 関連疾患Defects in MEF2A might be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1) [MIM:608320].
  • 配列類似性Belongs to the MEF2 family.
    Contains 1 MADS-box domain.
    Contains 1 Mef2-type DNA-binding domain.
  • 翻訳後修飾Constitutive phosphorylation on Ser-408 promotes Lys-403 sumoylation thus preventing acetylation at this site. Dephosphorylation on Ser-408 by PPP3CA upon neuron depolarization promotes a switch from sumoylation to acetylation on residue Lys-403 leading to inhibition of dendrite claw differentiation. Phosphorylation on Thr-312 and Thr-319 are the main sites involved in p38 MAPK signaling and activate transcription. Phosphorylated on these sites by MAPK14/p38alpha and MAPK11/p38beta, but not by MAPK13/p38delta nor by MAPK12/p38gamma. Phosphorylation on Ser-408 by CDK5 induced by neurotoxicity inhibits MEF2A transcriptional activation leading to apoptosis of cortical neurons. Phosphorylation on Thr-312, Thr-319 and Ser-355 can be induced by EGF.
    Sumoylation on Lys-403 is enhanced by PIAS1 and represses transcriptional activity. Phosphorylation on Ser-408 is required for sumoylation. Has no effect on nuclear location nor on DNA binding. Sumoylated by SUMO1 and, to a lesser extent by SUMO2 and SUMO3. PIASx facilitates sumoylation in postsynaptic dendrites in the cerebellar cortex and promotes their morphogenesis.
    Acetylation on Lys-403 activates transcriptional activity. Acetylated by p300 on several sites in diffentiating myocytes. Acetylation on Lys-4 increases DNA binding and transactivation (By similarity). Hyperacetylation by p300 leads to enhanced cardiac myocyte growth and heart failure.
    Proteolytically cleaved in cerebellar granule neurons on several sites by caspase 3 and caspase 7 following neurotoxicity. Preferentially cleaves the CDK5-mediated hyperphosphorylated form which leads to neuron apoptosis and transcriptional inactivation.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ADCAD1 antibody
    • MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A) antibody
    • MEF2 antibody
    • MEF2A antibody
    • MEF2A_HUMAN antibody
    • Myocyte enhancer factor 2A antibody
    • Myocyte-specific enhancer factor 2A antibody
    • RSRFC4 antibody
    • RSRFC9 antibody
    • Serum response factor like protein 1 antibody
    • Serum response factor-like protein 1 antibody
    see all

Anti-MEF2A (phospho S408) antibody 画像

  • This image shows human breast carcinoma tissue stained with ab51151 at 1/50 dilution. The cells in the right hand image were treated with the immunising phosphopeptide, the cells in the left hand image were not.

Anti-MEF2A (phospho S408) antibody (ab51151) 使用論文

ab51151 has not yet been referenced specifically in any publications.

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