製品の概要

  • 製品名Anti-MED6 antibody - ChIP Grade
    MED6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MED6 - ChIP Grade
  • アプリケーション適用あり: WB, ChIPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide within residues: LLLDLRQKFP PKFVQLKPGE KPVPVDQTKK EAEPIPETVK PEEKETTKNV, corresponding to internal sequence amino acids 180-229 of Human MED6 (NP_005457)

  • ポジティブ・コントロール
    • 721_B cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ChIP Use at an assay dependent concentration.

ターゲット情報

  • 機能Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • 配列類似性Belongs to the Mediator complex subunit 6 family.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activator recruited cofactor 33 kDa antibody
    • Activator-recruited cofactor 33 kDa component antibody
    • ARC33 antibody
    • hMed6 antibody
    • MED6 antibody
    • MED6_HUMAN antibody
    • Mediator complex subunit 6 antibody
    • Mediator of RNA polymerase II transcription subunit 6 antibody
    • NY REN 28 antigen antibody
    • Renal carcinoma antigen NY-REN-28 antibody
    • RNA polymerase transcriptional regulation mediator subunit 6 antibody
    see all

Anti-MED6 antibody - ChIP Grade 画像

  • Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30 minutes) or (0, 30, 60 minutes) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.

  • Anti-MED6 antibody - ChIP Grade (ab84968) at 1 µg/ml + 721_B cell lysate at 10 µg/ml

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 28 kDa
    Observed band size : 28 kDa

Anti-MED6 antibody - ChIP Grade (ab84968) 使用論文

ab84968 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab84968.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"