製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MED28
  • アプリケーション
    適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A 17 amino acid peptide from near the center of human MED28.

  • ポジティブ・コントロール
    • Human brain tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab73294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml.

ターゲット情報

  • 機能
    Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways.
  • 組織特異性
    Widely expressed. Highly expressed in vascular tissues such as placenta, testis and liver.
  • 配列類似性
    Belongs to the Mediator complex subunit 28 family.
  • 細胞内局在
    Nucleus. Cytoplasm. Membrane. According to PubMed:15467741, it is cytoplasmic and mainly membrane-associated.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EG1 antibody
    • Endothelial-derived protein 1 antibody
    • FKSG20 antibody
    • Magicin antibody
    • med28 antibody
    • MED28_HUMAN antibody
    • Mediator complex subunit 28 antibody
    • Mediator of RNA polymerase II transcription subunit 28 antibody
    • Merlin and Grb2-interacting cytoskeletal protein antibody
    • Tumor angiogenesis marker EG-1 antibody
    see all

画像

  • All lanes : Anti-MED28 antibody (ab73294) at 1 µg/ml

    Lane 1 : brain tissue lysate
    Lane 2 : brain tissue lysate with blocking peptide

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 20 kDa
    Observed band size : 20 kDa
  • ab73294 at 2.5µg/ml staining MED28 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

  • ICC/IF image of ab73294 stained HeLa cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73294, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

ab73294 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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