製品の概要

  • 製品名Anti-MED13L antibody
    MED13L 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to MED13L
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Dog, Chimpanzee, Ferret, Rhesus monkey, Orangutan, Elephant
  • 免疫原

    Synthetic peptide within Human MED13L aa 550-600. The exact sequence is proprietary. (GeneID 23389).
    Database link: NP_056150.1

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab87831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 243 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway.
  • 組織特異性Highly expressed in brain (cerebellum), heart (aorta), skeletal muscle, kidney, placenta and peripheral blood leukocytes. Highly expressed in fetal brain.
  • 関連疾患Defects in MED13L are a cause of transposition of the great arteries, dextro-looped (DTGA) [MIM:608808]. DTGA consists of complete inversion of the great vessels, so that the aorta incorrectly arises from the right ventricle and the pulmonary artery incorrectly arises from the left ventricle. This creates completely separate pulmonary and systemic circulatory systems, an arrangement that is incompatible with life. Patients often have atrial and/or ventricular septal defects or other types of shunting that allow some mixing between the circulations in order to support life minimally, but surgical intervention is always required.
    Note=A chromosomal aberration involving MED13L is found in a patient with transposition of the great arteries, dextro-looped and mental retardation. Translocation t(12;17)(q24.1;q21).
  • 配列類似性Belongs to the Mediator complex subunit 13 family.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MD13L_HUMAN antibody
    • MED13L antibody
    • Mediator complex subunit 13 like antibody
    • Mediator complex subunit 13-like antibody
    • Mediator of RNA polymerase II transcription subunit 13-like antibody
    • THRAP2 antibody
    • Thyroid hormone receptor-associated protein 2 antibody
    • Thyroid hormone receptor-associated protein complex 240 kDa component-like antibody
    • TRAP240L antibody
    see all

Anti-MED13L antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MED13L with ab87831 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-MED13L antibody (ab87831) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg/ml
    Lane 2 : HeLa whole cell lysate at 15 µg/ml
    Lane 3 : HeLa whole cell lysate at 5 µg/ml

    Developed using the ECL technique

    Predicted band size : 243 kDa


    Exposure time : 3 minutes
  • Detection of MED13L by Immunoprecipitation in whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab87831 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent WB detection. Chemiluminescence with an exposure time of 30 seconds.

Anti-MED13L antibody (ab87831) 使用論文

This product has been referenced in:
  • Tsai KL  et al. A conserved Mediator-CDK8 kinase module association regulates Mediator-RNA polymerase II interaction. Nat Struct Mol Biol 20:611-9 (2013). WB ; Human . Read more (PubMed: 23563140) »

See 1 Publication for this product

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