Anti-MED13/GCT 抗体 (ab76923)
Key features and details
- Rabbit polyclonal to MED13/GCT
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MED13/GCT antibody
MED13/GCT 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MED13/GCT -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Gorilla -
免疫原
Synthetic peptide within Human MED13/GCT aa 300-400. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_005112.2
Database link: Q9UHV7 -
ポジティブ・コントロール
- HeLa and 293T whole cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76923の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 240 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 240 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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関連性
MED13 is a member of the mediator complex that can act as both a positive and negative regulator of transcription. The mediator complex interacts with RNA pol II and its associated proteins, the general transcription factors. Mediator proteins serve as a bridge connecting the activator and basal transcription machinery. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 9969 Human
- Omim: 603808 Human
- SwissProt: Q9UHV7 Human
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別名
- 1110067M05Rik antibody
- Activator-recruited cofactor 250 kDa component antibody
- ARC250 antibody
see all
画像
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All lanes : Anti-MED13/GCT antibody (ab76923) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 240 kDa
Observed band size: 300 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Detection of MED13/GCT by Western Blot of Immunprecipitate.
ab76923 at 1µg/ml staining MED13/GCT in HeLa whole cell lysate immunoprecipitated using ab76923 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab76923 は 4 報の論文で使用されています。
- Tripathi S et al. Smad7:ß-catenin complex regulates myogenic gene transcription. Cell Death Dis 10:387 (2019). PubMed: 31097718
- Snijders Blok L et al. De novo mutations in MED13, a component of the Mediator complex, are associated with a novel neurodevelopmental disorder. Hum Genet N/A:N/A (2018). PubMed: 29740699
- Vijayalingam S & Chinnadurai G Adenovirus L-E1A activates transcription through mediator complex-dependent recruitment of the super elongation complex. J Virol 87:3425-34 (2013). Human . PubMed: 23302885
- Fukuda A et al. Heterogeneous nuclear ribonucleoprotein R cooperates with mediator to facilitate transcription reinitiation on the c-Fos gene. PLoS One 8:e72496 (2013). PubMed: 23967313