製品の概要

  • 製品名Anti-MED12L antibody
  • 製品の詳細
    Rabbit polyclonal to MED12L
  • アプリケーション適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    antigen sequence: LSVTASTRPR SPVGENADEH YSKDHDVKME IFSPMPGESC ENANTSLGRR MSVNCEKLVK REKPRELIFP SNYDLL, corresponding to amino acids 627-702 of Human MED12L

  • ポジティブ・コントロール
    • Human liver tissue

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab122368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能May be a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • 配列類似性Belongs to the Mediator complex subunit 12 family.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MD12L_HUMAN antibody
    • MED12L antibody
    • Mediator complex subunit 12-like protein antibody
    • Mediator of RNA polymerase II transcription subunit 12-like protein antibody
    • NOPAR antibody
    • Thyroid hormone receptor-associated-like protein antibody
    • TNRC11L antibody
    • TRALP antibody
    • TRALPUSH antibody
    • Trinucleotide repeat-containing gene 11 protein-like antibody
    see all

Anti-MED12L antibody 画像

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleoli. Recommended concentration of ab122368 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122368, at 1/100 dilution, staining MED12L in paraffin-embedded Human liver tissue by immunohistochemistry.

Anti-MED12L antibody (ab122368) 使用論文

ab122368 has not yet been referenced specifically in any publications.

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