Anti-MED12 抗体 (ab70842)
Key features and details
- Rabbit polyclonal to MED12
- Suitable for: ICC/IF, IHC-P, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MED12 antibody
MED12 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MED12 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, IP, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan -
免疫原
Synthetic peptide corresponding to Human MED12 aa 2150-2177 (C terminal). Immunogen derived form sequence corresponding to amino acids 2150 - C-terminus.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70842の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P | (1) |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IP |
Use at 2-5 µg/mg of lysate.
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WB | (2) |
1/2000 - 1/10000. Predicted molecular weight: 227 kDa.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IP
Use at 2-5 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 227 kDa. |
ターゲット情報
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機能
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. -
組織特異性
Ubiquitous. -
関連疾患
Defects in MED12 are the cause of Opitz-Kaveggia syndrome (OKS) [MIM:305450]; also known as FG syndrome type 1 (FGS1) or FG syndrome (FGS). OKS is an X-linked disorder characterized by mental retardation, relative macrocephaly, hypotonia and constipation.
Defects in MED12 are the cause of Lujan-Fryns syndrome (LUJFRYS) [MIM:309520]; also known as X-linked mental retardation with marfanoid habitus. Clinically, Lujan-Fryns syndrome can be distinguished from Opitz-Kaveggia syndrome by tall stature, hypernasal voice, hyperextensible digits and high nasal root. -
配列類似性
Belongs to the Mediator complex subunit 12 family. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 520974 Cow
- Entrez Gene: 480952 Dog
- Entrez Gene: 9968 Human
- Entrez Gene: 59024 Mouse
- Entrez Gene: 100157450 Pig
- Entrez Gene: 679693 Rat
- Omim: 300188 Human
- SwissProt: Q93074 Human
see all -
別名
- Activator recruited cofactor 240 kDa component antibody
- Activator-recruited cofactor 240 kDa component antibody
- ARC240 antibody
see all
画像
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All lanes : Anti-MED12 antibody (ab70842) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Lane 5 : Whole cell lysate from NIH 3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 227 kDa
Observed band size: 238 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MED12 with ab70842 at 1/1000 (1µg/ml). Detection: DAB.
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Detection of Human MED12 by Immunoprecipitation using Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70842 for IP at 3 µg/mg lysate. Subsequent WB detection was performed using ab70842 at 1 µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (11)
ab70842 は 11 報の論文で使用されています。
- Ren M et al. MED13 and glycolysis are conserved modifiers of α-synuclein-associated neurodegeneration. Cell Rep 41:111852 (2022). PubMed: 36543134
- Youn DY et al. The Mediator complex kinase module is necessary for fructose regulation of liver glycogen levels through induction of glucose-6-phosphatase catalytic subunit (G6pc). Mol Metab 48:101227 (2021). PubMed: 33812059
- Youn DY et al. The subunit assembly state of the Mediator complex is nutrient-regulated and is dysregulated in a genetic model of insulin resistance and obesity. J Biol Chem 294:9076-9083 (2019). PubMed: 31028171
- Xu M et al. MED12 exerts an emerging role in actin-mediated cytokinesis via LIMK2/cofilin pathway in NSCLC. Mol Cancer 18:93 (2019). PubMed: 31072327
- Tripathi S et al. Smad7:ß-catenin complex regulates myogenic gene transcription. Cell Death Dis 10:387 (2019). PubMed: 31097718